Crystal Structure of Cold-Active Alkaline Phosphatase from the Psychrophile Shewanella sp.

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概要

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• The crystal structure of a cold-active alkaline phosphatase from a psychrophile, Shewanella sp. (SCAP), was solved at 2.2 Å. A refined model showed a homodimer with six metal-ligand sites. The arrangement of the catalytic residues resembled those of alkaline phosphatases (APs), suggesting that the reaction mechanism of SCAP was fundamentally identical to those of other APs. SCAP had two distinct structural features: (i) a loop with Arg122 that bound to the phosphate moiety of the substrate suffered no constraints from the linkage to other secondary structures, and (ii) Mg3-ligand His109 was considered to undergo repulsive effect with neighboring Trp228. The local flexibility led by these features might be an important factor in the high catalytic efficiency of SCAP at low temperatures.

• 社団法人 日本農芸化学会の論文
• 2010-01-23

著者

• Mikami B

  Kyoto Univ. Kyoto Jpn

• Mikami Bunzo

  Graduate School Of Agriculture Kyoto Univ.

• Mikami Bunzo

  Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University

• AIZONO Yasuo

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Mikami B

  Kyoto Univ.

• TSURUTA Hiroki

  Office of Collaborative Research and Technology Development, Kobe University

• HIGASHI Tetsuo

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Graduate School of Agricu

• Aizono Y

  Laboratory Of Biological Chemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• Aizono Yasuo

  Lab. Of Biological Chemistry Dep. Of Biofunctional Chemistry Graduate School Of Agricultural Sci. Ko

• Aizono Yasuo

  Laboratory Of Biological Chemistry Department Of Biofunctinal Chemistry Faculty Of Agriculture Kobe

• Tsuruta H

  Kobe Univ. Hyogo

• Higashi Tetsuo

  Laboratory Of Applied Microbiology Department Of Applied Biological Chemistry Faculty Of Agriculture

• Higashi Tetsuo

  Laboratory Of Biological Chemistry Department Of Biofunctional Chemistry Graduate School Of Agricult

• Mikami Bunzo

  Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.

• Mikami Bunzo

  Laboratory of Applied Structural Biology, Division of Applied Life Science, Graduate School of Agriculture, Kyoto University

• Aizono Yasuo

  Laboratory of Biochemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

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