Two Sulfhydryl Groups Near the Active Site of Soybean β-Amylase
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概要
- 論文の詳細を見る
The less reactive SH groups of soybean β-amylase, SH4, SH5, and SH6, were modified with p-chloromercuribenzoic acid or N-ethylmaleimide, after the reactive SH groups, SH1, SH2, and SH3, were blocked with 5,5'-dithiobis-(2-nitrobenzoic acid) and cyanide. The enzyme activity decreased, accompanied by the modification of SH4. α-Cyclodextrin protected SH4 from the modification more effectively than maltose. The SH4-modified enzyme still bound to glucose, maltose, and α-cyclodextrin. SH4 was concerned with neither the catalysis nor substrate binding but its large substituent affected the substrate binding site. The sequencing of the 5-(iodoacetoamidoethyl)-aminonaphthalene-1-sulfonate-labeled peptides showed that SH4, SH5, and SH6 are Cys343, Cys82, and Cys208, respectively. Comparison of the primary structure of β-amylases also showed that the sequence around SH4 (Cys343), as well as SH2 (Cys95), is strongly conserved between higher plant and bacterial β-amylases. These results agree with the structure model deduced from X-ray crystallography of soybean β-amylase.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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Mikami B
Kyoto Univ. Kyoto Jpn
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Mikami Bunzo
Graduate School Of Agriculture Kyoto Univ.
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Mikami Bunzo
Research Institute For Food Science Kyoto University
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Morita Y
Japan Advanced Inst. Of Sci. And Technol. School Of Materials Sci. Ishikawa
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Morita Y
Kyoto Univ. Kyoto‐shi
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Morita Y
Toyama National Coll. Technol. Toyama Jpn
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Morita Yuhei
Research Institute For Food Science Kyoto University:(present Office)fuji Oil Co. Ltd. R. & D. C
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Morita Yuhei
Research Institute For Food Science Kyoto University
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Nomura Kohji
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
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Morita Y
Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Nomura Keiichi
Research Institute for Food Science, Kyoto University
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Yahiro Masatoshi
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Morita Yoshikazu
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Mikami B
Kyoto Univ.
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Nomura K
Kushimoto Marine Park Center Wakayama Jpn
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Mikami Bunzo
Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.
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