Cystatin C in Milk Basic Protein (MBP) and Its Inhibitory Effect on Bone Resorption in Vitro(Food & Nutrition Science)

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概要

• 論文の詳細を見る

• A cystein protease inhibitor was identified in the basic fraction of bovine milk. We have reported in our previous study that the milk basic protein (MBP) fraction suppressed osteoclast-mediated bone resorption in vitro. Since osteoclasts secreted cystein protease to digest collagen in the bone matrix, we identified the cystein protease inhibitor in MBP. A 12-kDa inhibitor was purified from MBP by papain affinity gel chromatography and subsequent Hi-Load Superdex 75 gel filtration chromatography. The N-terminal sequence of the 18 amino acid residues of the inhibitor corresponded to bovine cystatin C. The 12-kDa cystein protease inhibitor in MBP therefore seemed to be cystatin C. Purified cystatin suppressed bone resorption with the use of isolated osteoclasts in vitro. Cystatin in MBP is suggested as one of the factors inhibiting bone resorption.

• 社団法人日本農芸化学会の論文
• 2002-12-23

著者

• Toba Y

  Snow Brand Milk Products Co. Ltd. Saitama Jpn

• Toba Yasuhiro

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Takada Yukihiro

  雪印乳業技術研究所

• YAMAMURA Jun-ichi

  Technology and Research Institute, Snow Brand Milk Products Co., Ltd.

• MORITA Yoshikazu

  Technology and Research Institute, Snow Brand Milk Products Co., Ltd.

• TAKADA Yukihiro

  Technology and Research Institute, Snow Brand Milk Products Co., Ltd.

• KAWAKAMI Hiroshi

  Technology and Research Institute, Snow Brand Milk Products Co., Ltd.

• MATSUOKA Yasuhiro

  Technology and Research Institute, Snow Brand Milk Products Co. Ltd.

• SERIZAWA Atsushi

  Technology and Research Institute, Snow Brand Milk Products Co. Ltd.

• YOSHIOKA Tomoe

  Sapporo Research Laboratory, Snow Brand Milk Products Co. Ltd.

• TOBA Ysuhiro

  Technology and Research Institute, Snow Brand Milk Products Co. Ltd.

• KUMEGAWA Masayoshi

  Department of Oral Anatomy, Meikai University School of Dentistry

• Morita Y

  Japan Advanced Inst. Of Sci. And Technol. School Of Materials Sci. Ishikawa

• Morita Y

  Kyoto Univ. Kyoto‐shi

• Takada Y

  Snow Brand Milk Products Co. Ltd. Saitama Jpn

• Toba Y

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Takada Y

  Hokkaido Univ. Hokkaido Jpn

• Takada Yasuhiro

  Hokkaido Univ. Sapporo Jpn

• Yoshioka Tomoe

  Sapporo Research Laboratory Snow Brand Milk Products Co. Ltd.

• Takeuchi Yoji

  Cell Function And Structure Division Of Biological Sciences Graduate School Of Science Hokkaido Univ

• Yamamura Jun-ichi

  Department Of Virology Toyama Medical And Pharmaceutical University

• Yamamura Jun-ichi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Morita Y

  Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.

• Yahiro Masatoshi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Morita Yoshikazu

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Serizawa Atsushi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Kumegawa Masayoshi

  Department Of Oral Anatomy Josai Dental University

• Takeda Yuichi

  Fac. Sci. And Tech. Keio Univ. Yokohama

• Kawakami H

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Kawakami Hiroshi

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• TAKADA Yukihiro

  Technology and Research Institute, and Nutritional Science Laboratory

• TAKADA Yukihiro

  Technology & Research Institute, Snow Brand Milk Products Co., Ltd.

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