Purification and Characterization of a Cold-adapted Isocitrate Lyase and a Malate Synthase from Colwellia maris, a Psychrophilic Bacterium
スポンサーリンク
概要
- 論文の詳細を見る
Isocitrate lyase (ICL) and malate synthase (MS) of a psychrophilic marine bacterium, Colwellia maris, were purified to electrophoretically homogeneous state. The molecular mass of the ICL was found to be 240 kDa, composed of four identical subunits of 64.7 kDa. MS was a dimeric enzyme composed of 76.3 kDa subunits. N-Terminal amino acid sequences of the ICL and MS were analyzed. Purified ICL had its maximum activity at 20℃ and was rapidly inactivated at the temperatures above 30℃, but the optimum temperature for the activity of MS was 45℃. NaCl was found to protect ICL from heat inactivation above 30℃, but the salt did not stabilize MS. Effects of temperatures on the kinetic parameters of both the enzymes were examined. The K_m for the substrate (isocitrate) of ICL was decreased with decreasing temperature. On the other hand, the K_m for the substrate (glyoxylate) of MS was increased with decreasing temperature. The calculated value of free energy of activation of ICL was on the same level as that of MS.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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Takada Yasuhiro
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Toba Y
Snow Brand Milk Products Co. Ltd. Saitama Jpn
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Toba Yasuhiro
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Takada Yukihiro
雪印乳業技術研究所
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Takada Y
Snow Brand Milk Products Co. Ltd. Saitama Jpn
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Toba Y
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Takada Y
Hokkaido Univ. Hokkaido Jpn
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Takada Yasuhiro
Hokkaido Univ. Sapporo Jpn
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FUKUNAGA NORIYUKI
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Takeuchi Yoji
Cell Function And Structure Division Of Biological Sciences Graduate School Of Science Hokkaido Univ
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WATANABE Seiya
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Fukunaga Noriyuki
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Watanabe S
Nisshin Oil Mills Ltd. Tokyo Jpn
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Takeda Yuichi
Fac. Sci. And Tech. Keio Univ. Yokohama
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