Studies on the Inactivation of Protein Synthesis in Mesophilic Bacteria Induced by Chilling

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概要

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• The mechanism of inactivation of in vivo protein synthesis (incorporation of phenylalanine into protein at 20℃), by chilling at 0℃, in Escherichia coli Q13 and Pseudomonas aeruginosa was studied. In vitro protein synthesis with poly(U) or R17 phage RNA as mRNA showed that the protein-synthesizing system itself was not damaged by the chilling. In contrast, the ability of E. coli Q13 and P. aeruginosa to take up phenylalanine decreased by 100% and 90%, respectively, after the 8-day chilling period. A significant part of the phenylalanine pool leaked out of the cells during the chilling period. Intracellular ATP levels and the energy balance did not alter greatly in E. coli Q13, but changed considerably in P. aeruginosa due to chilling. These results strongly suggest that the inactivation of the in vivo protein synthesis is due to damage of membrane functions, probably those related to the amino acid transport system, and not to the inactivation of the protein-synthesizing system itself.

• 日本植物生理学会の論文

著者

• Sasaki Shoji

  Department of Biology, Faculty of Science, Hokkaido University

• Fukunaga Noriyuki

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Fukunaga Noriyuki

  Department Of Biology Faculty Of Science Hokkaido University

• Sasaki S

  Tohoku Univ. Snedai Jpn

• Saruyama Haruo

  Department Of Biology Faculty Of Science Hokkaido University

• Sasaki Shoji

  Department Of Biology Faculty Of Science Hokkaido University

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