Changes in Lipoxygenase Components of Rice Seedlings during Germination

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概要

• 論文の詳細を見る

• Changes in lipoxygenase (LOX) activity were followed during the germination of rice seeds. The enzyme activity of 3-day-old seedlings was 20 times higher than that of ungerminated seeds. Sixty per cent of the increased activity was found in shoots. The increase in LOX activity was mainly due to an increase in lipoxygenase-2 (LOX-2), a minor component in ungerminated seeds ; this increase was inhibited by cycloheximide. LOX-2 was isolated from the 3-day-old seedlings and compared for its enzymological properties with rice lipoxygenase-3 (LOX-3), a major component in ungerminated seeds. Both LOX-2 and LOX-3 were stable at pH 5 to 8, but LOX-2 was more heat-stable than LOX-3. Apparent K_m values of LOX-2 and LOX-3 for linoleic acid were 170 and 59 μM, and those for linolenic acid were 5,300 and 88 μM, respectively. Both LOXs were inhibited by some metal ions and antioxidants.

• 日本植物生理学会の論文

著者

• Mikami B

  Kyoto Univ. Kyoto Jpn

• Mikami Bunzo

  Graduate School Of Agriculture Kyoto Univ.

• Mikami Bunzo

  Research Institute For Food Science Kyoto University

• Morita Y

  Univ. Osaka Prefecture Osaka Jpn

• Morita Y

  Kyoto Univ. Kyoto‐shi

• Morita Yuhei

  Research Institute For Food Science Kyoto University:(present Office)fuji Oil Co. Ltd. R. & D. C

• Morita Yuhei

  Research Institute For Food Science Kyoto University

• OHTA Hiroyuki

  Research Institute for Food Science, Kyoto University

• IDA Shoji

  Research Institute for Food Science, Kyoto University

• Ida S

  Kyoto Univ. Kyoto Jpn

• Ida Shoji

  Research Institute For Food Science Kyoto University

• Mikami B

  Kyoto Univ.

• Ohta Hiroyuki

  Research Institute For Food Science Kyoto University

• Mikami Bunzo

  Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.

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