Fractionation of Isozymes and Determination of the Subsite Structure of Glucoamylase from Rhizopus niveus
スポンサーリンク
概要
- 論文の詳細を見る
Four isozymes of glucoamylase were isolated from a commercial preparation of the enzyme from Rhizopus niveus by ion-exchange chromatography on CM-Sephadex C-50 and/or the preparative isoelectric focusing method on Sephadex IEF. The kinetic parameters, Michaelis constant Km and molecular activity k0, for the hydrolysis of maltooligosaccharides (degree of polymerization DP from 2 to 7) were determined on the major component enzyme at pH 4.5 and 25°C. It was found that Km decreases and k0 increases with increasing DP. From the DP-dependences of Km and k0, the subsite affinities of the seven subsites of the enzyme active site were evaluated on the basis of subsite theory. These values are very similar to those obtained for an unfractionated preparation of Rhizopus delemar glucoamylase [Hiromi et al, B. B. A., 302, 362 (1973)].
- 社団法人 日本農芸化学会の論文
著者
-
Aibara S
Research Institute For Food Science Kyoto University
-
Aibara Shigeo
Research Institute For Food Science Kyoto University
-
Morita Yuhei
Research Institute For Food Science Kyoto University:(present Office)fuji Oil Co. Ltd. R. & D. C
-
Morita Yuhei
Research Institute For Food Science Kyoto University
-
HIROMI Keitaro
Department of Food Science and Technology, Faculty of Engineering, Fukuyama University
-
TANAKA Akiyoshi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
-
FUKUCHI Yoshikazu
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
-
OHNISHI Masatake
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
-
Hiromi Keitaro
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
-
Tanaka Akiyoshi
Department Of Chemistry Faculty Of Education Mie University
-
HIROMI Keitaro
Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
関連論文
- Protein crystallization in microgravity
- Protein Crystallization in Microgravity (特集:日本の宇宙生物学実験)
- Purification and Characterization of Rice Lipoxygenase Component 3 from Embryos(Biological Chemistry)
- Changes in Lipoxygenase Components of Rice Seedlings during Germination
- The Occurrence of Lipid Hydroperoxide-Decomposing Activities in Rice and the Relationship of Such Activities to the Formation of Antifungal Substances
- Post-harvest Drying of Fresh Rice Grain and Its Effects on Deterioration of Lipids during Storage(Food & Nutrition)
- Changes in Rice Bran Lipids and Free Amino Acids during Storage
- Post-harvest Changes in the Respiratory Activity of and Ethanol Accumulation in Fresh Rice Grains
- Reconstitution of Arachin from Isolated Subunits
- Positions of Substituted Amino Acids in Soybean β-Amylase Isozymes(Biological Chemistry)
- Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Microbiology & Fermentation Industry)
- Characterization of β-amylase isozymes in soybean seeds by isoelectric focusing
- Effects of High-voltage Electric Field Treatment on the Water Activity of Bread
- Bilirubin Oxidase from Trachyderma tsunodae K-2593,a Multi-copper Enzyme
- Characterization of Monoclonal Antibodies against (1R, 2R)-Cyclohexanediamine Platinum(II)-DNA Adduct
- Fractionation of Isozymes and Determination of the Subsite Structure of Glucoamylase from Rhizopus niveus
- Two Sulfhydryl Groups Near the Active Site of Soybean β-Amylase
- Lysyl-tRNA Synthetase from Bacillus stearothermophilus. Formation and Isolation of an Enzyme・Lysyladenylate Complex and Its Analogue^1
- Lysyl-tRNA Synthetase from Bacillus stearothermophilus. Purifiation, and Fluorometric and Kinetic Analysis of the Binding of Substrates, L-Lysine and ATP^1
- Contributions of Polysaccharide and Lipid Regions of Lipopolysaccharide to the Recognition by Spike G Protein of Bacteriophage φX174(Biochemistry & Molecular Biology)
- Steady-state Inhibitory Kinetic Studies on Almond β-Glucosidase-catalyzed Reaction : Binding Sites of Monosaccharides(Biological Chemistry)
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Purification and Characterization ofα-Glucosidase from Torulaspora pretoriensis YK-1
- CHARACTERIZATION OF BOVINE SUPEROXIDE DISMUTASE AND ITS MUTANTS
- pH-Jump of Pepsin Studied by Tyrosine Ionization(Biological Chemistry)
- Effects of High-Voltage Electric Field Treatment on Bread Starch
- Process of Thermal Denaturation of Xylanase (XynB) from Clostridium stercorarium F-9
- Differential Scanning Calorimetric Studies on the Domain Structure of Aspergillus Glucoamylase^1
- Characterization of the Binding of Spike H Protien of Bacteriophage ΦX174 with Receptor Lipopolysaccharides
- Effect of Denervation of the Pylorus and Transection of the Duodenum on Acetaminophen Absorption in Rats : Possible Mechanism for Early Delayed Gastric Emptying after Pylorus Preserving Pancreatoduodenectomy
- Molecular Shape and Packing in Crystals of Soybean β-Amylase
- Valyl-tRNA synthetase from Bacillus stearothermophilus. Purification and Binding with the Substrates L-Valine and ATP(Biological Chemistry)
- Subsite Structure of Rhizopus Glucoamylase for the Hydrolytic Reaction of Isomaltooligosaccharides
- Mechanical properties of the femur after injection of calcium phosphate cement containing arbekacin sulfate and polylactic acid in a rat model of experimental osteoporosis
- Steady-State Kinetic and Calorimetric Studies on the Binding of Aspergillus niger Glucoamylase with Gluconolactone, 1-Deoxynojirimycin, and β-Cyclodextrin
- Random Attack as the Hydrolytic Reaction Mode of Oligosaccharides by Rhizopus Glucoamylase
- Differential Scanning Calorimetric Study of the Thermal Denaturation of Almond β-Glucosidase(Biological Chemistry)
- Spectrophotometric Determination of Protein by the Stopped-flow Time Difference Analysis Technique to Enhance the Sensitivity of the Coomassie Brilliant Blue-binding Method(Analytical Chemistry)
- Application of the "Micro-Stopped-Flow Time Difference Analysis" Technique to a Dehydrogenase-Catalyzed Reaction for Rapid and Sensitive Determination of Pyruvate(Analytical Chemistry)
- Static and kinetic studies of the interaction of tryptophan with fluorescein mercury(II) acetate by fluorescence and absorption spectrophotometry and by fluorescence stopped-flow method.
- Rapid Preparation of Maltooligosaccharides from Cyclodextrins by Column Chromatography of Hydrophilic Vinyl Polymer Gel
- Inhibitory Spectrum of Talopeptin (MKI), a Specific Inhibitor of Thermolysin
- Characterization of the Binding of Spike H Protein of Bacteriophage .PHI.X174 with Receptor Lipopolysaccharides.
- Inhibition of L-Glycerol 3-Phosphate Acyltransferase from Escherichia coli by cis-9, 10-Methylenehexadecanoic Acid
- N-terminal sequence of soybean .BETA.-amylase.