Inhibitory Spectrum of Talopeptin (MKI), a Specific Inhibitor of Thermolysin
スポンサーリンク
概要
- 論文の詳細を見る
Talopeptin (N-(6-deoxy-α-L-talopyranosyloxyphospho)-L-leucyl-L-tryptophan), a specific inhibitor for thermolysin and some other endo-type metalloproteases, does not inhibit superoxide dismutase, alkaline phosphatase, or carbonic anhydrase, which are Zn(II)-enzymes, in the inhibitor concentration of the order of 10-4M. Although the enzyme activity of carboxypeptidase A, a Zn(II)-containing exo-type metalloprotease, is slightly reduced by the addition of talopeptin, the inhibition is not significant at the 10% leveljudged by Students t test. On the other hand, talopeptin seemed to act as an inhibitor for all dehydrogenases examined, most of which do not contain Zn(II), at the inhibitor concentration studied (around 10-4m). It is possible that the phosphite group of talopeptin binds to the same site on the dehydrogenase as the one for the phosphate group of the coenzyme.
- 社団法人 日本農芸化学会の論文
著者
-
KITAGISHI Keiko
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
-
HIROMI Keitaro
Departmentof Food Science and Technology, Faculty of Agriculture, Kyoto University
-
HIROMI Keitaro
Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
関連論文
- Bilirubin Oxidase from Trachyderma tsunodae K-2593,a Multi-copper Enzyme
- Characterization of Monoclonal Antibodies against (1R, 2R)-Cyclohexanediamine Platinum(II)-DNA Adduct
- Fractionation of Isozymes and Determination of the Subsite Structure of Glucoamylase from Rhizopus niveus
- Lysyl-tRNA Synthetase from Bacillus stearothermophilus. Formation and Isolation of an Enzyme・Lysyladenylate Complex and Its Analogue^1
- Lysyl-tRNA Synthetase from Bacillus stearothermophilus. Purifiation, and Fluorometric and Kinetic Analysis of the Binding of Substrates, L-Lysine and ATP^1
- Novel β-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25
- Purification and Characterization ofα-Glucosidase from Torulaspora pretoriensis YK-1
- CHARACTERIZATION OF BOVINE SUPEROXIDE DISMUTASE AND ITS MUTANTS
- pH-Jump of Pepsin Studied by Tyrosine Ionization(Biological Chemistry)
- Valyl-tRNA synthetase from Bacillus stearothermophilus. Purification and Binding with the Substrates L-Valine and ATP(Biological Chemistry)
- Spectrophotometric Determination of Protein by the Stopped-flow Time Difference Analysis Technique to Enhance the Sensitivity of the Coomassie Brilliant Blue-binding Method(Analytical Chemistry)
- Application of the "Micro-Stopped-Flow Time Difference Analysis" Technique to a Dehydrogenase-Catalyzed Reaction for Rapid and Sensitive Determination of Pyruvate(Analytical Chemistry)
- Static and kinetic studies of the interaction of tryptophan with fluorescein mercury(II) acetate by fluorescence and absorption spectrophotometry and by fluorescence stopped-flow method.
- Rapid Preparation of Maltooligosaccharides from Cyclodextrins by Column Chromatography of Hydrophilic Vinyl Polymer Gel
- Inhibitory Spectrum of Talopeptin (MKI), a Specific Inhibitor of Thermolysin