Application of the "Micro-Stopped-Flow Time Difference Analysis" Technique to a Dehydrogenase-Catalyzed Reaction for Rapid and Sensitive Determination of Pyruvate(Analytical Chemistry)
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概要
- 論文の詳細を見る
The great usefulness of the previously reported "stopped-flow time difference analysis" technique for improving enzymatic analyses based on dehydrogenase-catalyzed reactions is demonstrated by combining the technique with the conventional enzymatic method for pyruvate determination. Reagent economy was accomplished by using a micro-stopped-flow apparatus designed by one of the authors. The running speed and sensitivity were improved by about 100 and 7 times the figures for the conventional enzymatic method, respectively. Furthermore, as procedural blanks were unnecessary, a simpler method could be developed. The interference by several other keto acids was easily eliminated by analyzing the reaction curve, and the apparent first-order rate constant proved to be useful for qualitative analysis.
- 社団法人日本農芸化学会の論文
- 1988-10-23
著者
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Hiromi Keitaro
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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KANAYA Ken-ichiro
Japan Food Research Laboratories, Osaka Branch
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Kanaya K
Japan Food Research Laboratories Osaka Branch
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Kanaya Ken-ichiro
Japan Food Research Laboratories Osaka Branch
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HIROMI Keitaro
Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
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