Purification, Characterization, and Crystallization of Single Molecular Species of β-Conglycinin from Soybean Seeds

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概要

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• Four major molecular species of β-conglycinin, α_3, α_2β, αβ_2, andβ_3, were isolated and purified from seeds of an α' subunit-deficient strain of soybeans (Glycine max). All components were found to be homogeneous by high pressure liquid chromatography, SDS-polyacrylamide gel electrophoresis, and amino acid and amino terminal sequence analyses. The amino acid compositions of the α_3 and β_3 components agreed fairly well with the compositions deduced from the cDNA sequences, and all of the components were highly glycosylated. The α_3 and β_3 components were compared regarding their secondary structures. The secondary structure of the α_3 component deduced from CD measurements showed a higher α-helix content than that of the β_3 component. The β_3 component was crystallized by decreasing the ionic strength from 0.5 to 0.14 in phosphate buffer, pH 7.3, and the crystals grew to a size (1.0 mm×0.2 mm×0.2 mm) suitable for X-ray crystallographic analysis. A preliminary X-ray analysis showed that the crystal belonged to an orthorhombic crystal system having the space group P2_12_12_1 and unit cell dimensions of a= 185.1 Å, b = 107.9 Å, and c = 97.6 Å.

• 社団法人日本農芸化学会の論文
• 1996-05-23

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Fukuda Y

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Morita Y

  Japan Advanced Inst. Of Sci. And Technol. School Of Materials Sci. Ishikawa

• Morita Y

  Kyoto Univ. Kyoto‐shi

• Yamada Makiko

  San-ei Gen F.f.i. Inc.

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• MORITA Shimpei

  Central Research Institute, Fuji Oil Co.

• FUKASE Masami

  Central Research Institute, Fuji Oil Co.

• MORITA Yuhei

  Central Research Institute, Fuji Oil Co.

• YAMAGUCHI Masami

  Central Research Institute, Fuji Oil Co.

• FUKUDA Yoichi

  Central Research Institute, Fuji Oil Co.

• Morita Y

  Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.

• Yahiro Masatoshi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Morita Yoshikazu

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Yamaguchi Masayo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Fukase Masami

  Central Research Institute Fuji Oil Co.

• Yamaguchi M

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamaguchi M

  Murata Manufacturing Co. Ltd.

• Yamaguchi Magoichi

  Research Institute Nihon Shokuhin Kako Co. Ltd.

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