Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice : Oryza Sativa L.

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概要

• 論文の詳細を見る

• We have isolated a cDNA(RGRC2) encoding glutathione reductase(GR) from rice(Oryza sativa L.). The comparison of deduced amino acid sequences from RGRC2 and other plant GR cDNAs indicated that RGRC2 encodes a putative cytosolic isoform. The recombinant RGRC2 protein had enzymatic properties comparable to those of GR from rice embryo. Subcellular fractionation showed that the RGRC2 protein is localized primarily in cytosol. mRNA and protein of RGRC2 were observed mainly in roots and calli but little in leaf tissues. Southern blot analysis showed that the RGRC2 gene exists as a single copy gene. Here, we have also isolated a genomic clone completely corresponding to RGRC2. The RGRC2 gene is split into 16 exons spread about 7.4kb of chromosomal DNA, with coding sequence beginning in the 2nd exon and ending in the 16th exon. From the presence of two ABA-responsive elements in the 5'-flanking region of RGRC2, we examined the expression in rice seedlings treated with ABA and the ABA-related environmental stresses, chilling, drought and salinity. The expression of RGRC2 was strongly induced by all these treatments. We suggest that the expression of the rice cytosolic GR gene is regulated via ABA-mediated signal transduction pathway under environmental stresses.

• 日本植物生理学会の論文

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MORITA Shigeto

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MASUMURA Takehiro

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• Kaminaka Hironori

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• Nakajima Mieko

  Laboratory of Genetic Engineering, Faculty of Agriculture

• NAKAJIMA Mieko

  Lab. Genetic Engineering., Dept. Agri., Kyoto Pref. Univ.

• Kaminaka Hironori

  Lab. Genetic Engineering Fac. Of Agr. Kyoto Pref. Univ.

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Masumura T

  Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Nakajima Mieko

  Lab. Genetic Engineering. Dept. Agri. Kyoto Pref. Univ.

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• MORITA Shigeto

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

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