Molecular Cloning, Nucleotide Sequencing, and Regulation of the chiA Gene Encoding One of Chitinases from Enterobacter sp. G-1
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概要
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The chiA gene encoding a chitinase of 60-kDa has been cloned from Enterobacter sp. G-1 by PCR using synthetic oligonucleotides corresponding to the amino acid sequences of the purified enzyme and subsequently genomic library screening was performed. The products of the positive clones were found to degrade waterinsoluble chitin. The primary structure of the chiA gene consisted of 1,686-bp encoding 562 amino acid residues. Comparison of the deduced amino acid sequence of the cloned chitinase gene product (chiA) with other chitinases revealed a high homology (95.7% identity) with chitinase A from Serratia marcescens QMB1466. The coding region of the chiA gene for higher expression in Escherichia coli was identified using deletion and sequence analysis. The expression of the chiA gene in Enterobacter sp. G-1 was controlled by presence of chitin, as determined by Northern blotting hybridization analysis. We found that the expression of the chiA gene in E. coli was controlled by an inverted repeat sequence located in the upstream region from a promoter region.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
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Park Jae
Department of Nuclear and Energy Engineering, Jeju National University
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Takahashi N
Plant Functions Laboratory Riken
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OKAMOTO TAKASHI
Department of Pediatrics, Takamatsu Red Cross Hospital
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NAKAGAWA Tsuyoshi
Research Institute of Molecular Genetics, Shimane University
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MATSUDA Hideyuki
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Tanaka Katsunori
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Park Jae
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science
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YAMASAKI YUKIKAZU
Shimane University, and Institute of Industrial Science and Technology in Shimane Prefecture
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KAWAMUKA MAKOTO
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science
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YAMASAKI Yukikazu
Institute of Industrial Science and Technology in Shimane Prefecture
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Yamasaki Yukikazu
Shimane Institute For Industrial Technology
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Takahashi Nobuyuki
Hokkaido University Of Education
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Okamoto Takashi
Department Of Biological Sciences Tokyo Metropolitan University
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Matsuda H
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Matsuda Hideyuki
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Okamoto Takashi
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science
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Okamoto Takashi
Department Of Animal Products National Institute Of Livestock And Grassland Science
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Okamoto Takashi
Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University
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Park Jae
Department of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Kyunggi-do 440-746, South Korea
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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