Efficient Production of Recombinant Human (Pro)renin Utilizing a Decahistidine Tag Attached at the C-Terminus

スポンサーリンク

概要

• 論文の詳細を見る

• Human prorenin attached by a decahistidine tag at the C-terminus was produced in Chinese hamster ovary cells. The tagged protein secreted into the culture medium was in the inactive prorenin form, and was activated to mature renin by proteolytic removal of its prosegment by trypsin in the same manner as native prorenin. The tagged (pro)renin was efficiently purified by metal-chelate affinity chromatography. The enzymatic properties of mature renin carrying the tag were similar to native renin. These results indicate that the introduction of a decahistidine tag at the C-terminus does not interfere with either the correct folding of prorenin or the catalytic activity of mature renin.

• 社団法人 日本農芸化学会の論文
• 2007-01-23

著者

• Suzuki Fumiaki

  Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University

• Nakamura Yoshimasa

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Takahashi N

  Plant Functions Laboratory Riken

• Suzuki F

  Laboratory Of Animal Biochemistry Faculty Of Applied Biological Science Gifu University

• Suzuki F

  Gifu Univ Gifu Jpn

• Suzuki Fumiaki

  Laboratory Of Animal Biochemistry Faculty Of Applied Biological Sciences Gifu University

• Suzuki Fumiaki

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• Nakano Takeshi

  Riken Advanced Sci. Inst.

• Nakagawa Tetsuto

  Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra

• Nakagawa Tsuyoshi

  Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.

• Nakamura Yukio

  Laboratory Of Organic Chemistry Faculty Of Technology Kanagawa University

• NAKANO Takeshi

  The Institute of Physical and Chemical Research (RIKEN)

• Suzuki Fumiaki

  United Graduate School Of Agricultural Science Gifu University

• Nakagawa T

  Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra

• IWATA Hideyuki

  United Graduate School of Agricultural Science, Gifu University

• NAKAGAWA Tsutomu

  Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University

• NISHIUCHI Kazuhiro

  Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University

• AKAKI Jyunji

  Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University

• SATOU Ryousuke

  United Graduate School of Agricultural Science, Gifu University

• Ido Hiroki

  United Graduate School Of Agricultural Science Gifu University

• Takahashi Nobuyuki

  Hokkaido University Of Education

• Akaki Jyunji

  Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University

• Satou Ryousuke

  United Graduate School Of Agricultural Science Gifu University

• Nakano Takeshi

  Plant Functions Lab.

• Nishiuchi Kazuhiro

  Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University

• NAKAMURA YUKIO

  Laboratory of Animal Reproduction, College of Agriculture, University of Osaka Prefecture

• NAKAGAWA Tsutomu

  Laboratory of Animal Biochemistry, United Graduate School of Agricultural Science, Gifu University

関連論文

• Biochemical properties of renin and prorenin binding to the (pro)renin receptor
• Binding Affinity of Full-length and Extracellular Domains of Recombinant Human (Pro)renin Receptor to Human Renin When Expressed in the Fat Body and Hemolymph of Silkworm Larvae.
• An Avocado Constituent, Persenone A, Suppresses Expression of Inducible Forms of Nitric Oxide Synthase and Cyclooxygenase in Macrophages, and Hydrogen Peroxide Generation in Mouse Skin
• Inhibition by (-)-Persenone A-related Compounds of Nitric Oxide and Superoxide Generation from Inflammatory Leukocytes
• Inhibitory Effects of Curcumin and Tetrahydrocurcuminoids on the Tumor Promoter-induced Reactive Oxygen Species Generation in Leukocytes in vitro and in vivo
• Expression and purification of human (pro)renin receptor in insect cells using baculovirus expression system
• ISOLATION OF BRASSINOSTEROID DEFICIENCY-INSENSITIVE MUTANTS OF ARABIDOPSIS
• DEVELOPMENT OF SELECTIVE AND POTENT BRASSINOSTEROID BIOSYNTHESIS INHIBITORS AND ISOLATION OF INHIBITOR INSENSITIVE MUTANTS OF ARABIDOPSIS
• Screnning of cytokinin biosynthesis inhibitors
• A novel albino mutant, alth deficient in chloroplast gene expression.
• Analysis of the Decaprenyl Diphosphate Synthase (dps) Gene in Fission Yeast Suggests a Role of Ubiquinone as an Antioxidant^1
• Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli
• Synthesis and Tumor-promoting Activities of 12-Epi-phorbol-12,13-dibutyrate
• Synthesis and Biological Activities of (-)-6-n-Octyl-indolactam-V, a New Potent Analogue of the Tumor Promoter (-)-Indolactam-V
• Synthesis and Biological Activities of Indolactone-V, the Lactone Analogue of the Tumor Promoter (-)-Indolactam-V
• Photocytotoxicity of Water-soluble Fullerene Derivatives
• Identification of the Female Sex Pheromone of the Cabbage Webworm
• Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
• Antimutagenic and Anticarcinogenic Properties of Kyo-yasai, Heirloom Vegetables in Kyoto
• (Pro)Renin Receptor-Mediated Activation of Mitogen-Activated Protein Kinases in Human Vascular Smooth Muscle Cells
• Kurosu, a Traditional Vinegar Produced from Unpolished Rice, Suppresses Lipid Peroxidation in Vitro and in Mouse Skin
• Gateway vectors for plant transformation
• Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
• P-38 ANALYSIS OF CH/π INTERACTION BETWEEN TUMOR PROMOTERS AND PROTEIN KINASE C C1 DOMAINS
• Binding Selectivity of Conformationally Restricted Analogues of (-)-Indolactam-V to the C1 Domains of Protein Kinase C Isozymes(Organic Chemistry)
• Selective Binding of Tumor Promoters to the Novel PKC C1B Domains
• Isolation of (-)-14-O-Malonylindolactam-V as a Possible Precursor of (-)-Indolactam-V and (-)-14-O-Acetylindolactam-V from Streptomyces blastmyceticum
• Inhibition of Pancreatic Carcinogenesis by Shark Cartilage Proteoglycan in Hamsters
• Evidence for the Existence of a Soybean Resistant Protein That Captures Bile Acid and Stimulates Its Fecal Excretion
• Simple and rapid chromatographic purification of type V collagen from a pepsin digest of porcine intestinal connective tissue, an unmanageable starting material for conventional column chromatography
• DNA Repair Effect of Traditional Sweet Pepper Fushimi-togarashi : Seen in Suppression of UV-induced Cyclobutane Pyrimidine Dimer in Human Fibroblast
• Inhibition of Prostaglandin Biosynthesis by 3-Nitro-2,4,6-trihydroxybenzamides
• Inhibition of Human 5-Lipoxygenase by 3-Nitro-2,4,6-trihydroxybenzamide Derivatives(Biological Chemistry)
• Structure-Activity Relationship of 3-Nitro-2,4,6-trihydroxybenzamide Derivatives in Photosynthetic Electron Transport Inhibition(Pesticide Chemistry)
• Syntheses of 3-Nitro-2,4,6-trihydroxy-benzamide and Thiobenzamide Derivatives as Highly Potent Inhibitors of Photosynthetic Electron Transport(Pesticide Chemistry)
• Inhibition of Photosynthesis by 3-Acyl-2,4,6-trihydroxythiobenzamide Derivatives(Organic Chemistry)
• Photosynthetic Electron Transport Inhibition by 3-Acyl-2, 4, 6-trihydroxybenzamide Derivatives(Organic Chemistry)
• Biological Activity of Nitrophlorophenone Derivatives
• Photosynthetic Electron Transport Inhibition by Phlorophenone Derivatives
• Synthesis and Activity of 3-(1-Alkylaminoalkylidene)-2H-pyran-2, 4(3H)-diones as New Photosynthetic Electron Transport Inhibitors
• Brassinolide-related New Bioactive Steroids from Dolichos lablab Seed
• Structures of the Metabolites from Steviol Methyl Ester by Gibberella fujikuroi
• Metabolism of Steviol and Its Derivatives by Gibberella fujikuroi
• Tyrosine-83 of Human Renin Contributes to Biphasic pH Dependence of the Renin-Angiotensinogen Reaction
• Non-proteolytic Activation of Human Prorenin by Anti-prorenin Prosegment (pf#1 : 1P-15P) Antiserum
• Feedback-regulated expression of brassinosteroid biosynthetic gene DWF4 in Arabidopsis
• Effects of Brassinolide and a specific inhibitor of brassinosteroid biosynthesis, Brassinazole on growth of Arabidopsis seedlings
• MOLECULAR CLONING OF ARABIDOPSIS GENES INVOLVED IN ROSETTE LEAF FORMATION BY DIFFERENTIAL DISPLAY TECHNIQUE
• Cytokinin inducible genes in culture green tobacco cells.
• Analysis of phyotohormone contents in tobacco CND41 antisense transformants.
• Analyzing the function of a 41 kD chloroplast-nucleoid DNA-binding protein (CND41) by F2 progeny of antisense-transgenic tobacco
• A CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN AND ITS FUNCTION IN CHLOROPLAST GENE EXPRESSION
• Purification, Characterization and Gene Analysis of N-Acetylglucosaminidase from Enterobacter sp. G-1
• Molecular Cloning, Nucleotide Sequencing, and Regulation of the chiA Gene Encoding One of Chitinases from Enterobacter sp. G-1
• Purification and Characterization of the Chitinase (ChiA) from Enterobacter sp. G-1
• Identification of Brassinosteroids with Epimerized Substituents and/or the 23-Oxo Group in Pollen and Anthers of Japanese Cedar
• Cloning and Sequencing Analysis of Alginate Lyase Genes from the Marine Bacterium Vibrio sp. O2
• Effects of Fucoidan from Mozuku on Human Stomach Cell Lines
• Apoptosis-inducing Activity of High Molecular Weight Fractions of Tea Extracts
• Genetic Analysis of chs1^+ and chs2^+ Encoding Chitin Synthases from Schizosaccharomyces pombe
• POSSIBLE ROLE OF CND41 IN DEGRADATION OF RUBISCO
• Accumulation of CND41 and expression of chloroplastic genes in cultured tobacco cell
• Protease activity of CND41, a chloroplast DNA-binding protein
• Functinal analysis of cig (cytokinin inducible gene)
• PURIFICATION AND CHARACTERIZATION OF CND41 FROM CULTURED TOBACCO CELL
• Analysing the function of a 41 kD chloroplast-nucleoid DNA-binding protein (CND41) by cultured cells from antisense-transgenic tobacco
• Apoptosis-inducing Activity of Polyphenol Compounds Derived from Tea Catechins in Human Histiolytic Lymphoma U937 Cells
• Two Glutamic Acids in Chitosanase A from Matsuebacter chitosanotabidus 3001 Are the Catalytically Important Residues
• Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal-and Organ-Specific Expression
• The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen
• Ser84 of Human Renin Contributes to the Biphasic pH Dependence of the Renin-Angiotensinogen Reaction
• Efficient Production of Recombinant Human (Pro)renin Utilizing a Decahistidine Tag Attached at the C-Terminus
• Angiotensin III as Well as Angiotensin II Regulates Water Flow through Aquaporins in a Clam Worm
• Effects of Substitution of Val for Leu^ of Oive Angiotensinogen on Renin Activity
• The Arabidopsis FLAKY POLLEN1 Gene Encodes a 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Required for Development of Tapetum-Specific Organelles and Fertility of Pollen Grains
• Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in Rice
• Structure of GDP/GTP exchange factor homolog which expression was induced by cytokinin in cultured green tobacco cells
• Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
• Improved Gateway Binary Vectors : High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
• REGULATION OF PROLYL ISOMERASE GENE EXPRESSION IN ARABIDOPSIS
• CHARACTERIZATION OF CYCLOPHILIN FROM ARABIDOPSIS THALIANA
• Exogenous Proline and Glycinebetaine Suppress Apoplastic Flow to Reduce Na^+ Uptake in Rice Seedlings
• Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
• Feedback-Regulation of Strigolactone Biosynthetic Genes and Strigolactone-Regulated Genes in Arabidopsis
• The Involvement of Intracellular Glutathione in Methyl Jasmonate Signaling in Arabidopsis Guard Cells
• Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
• Aliskiren binds to renin and prorenin bound to (pro)renin receptor in vitro
• The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen
• Ser84 of Human Renin Contributes to the Biphasic pH Dependence of the Renin-Angiotensinogen Reaction
• Efficient Production of Recombinant Human (Pro)renin Utilizing a Decahistidine Tag Attached at the C-Terminus
• Development of a Series of Gateway Binary Vectors Possessing a Tunicamycin Resistance Gene as a Marker for the Transformation of Arabidopsis thaliana
• The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane
• Surface Melting of an n-Alkane Crystallite
• Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in Arabidopsis thaliana
• The Roles of CATALASE2 in Abscisic Acid Signaling in Arabidopsis Guard Cells
• Expression Analysis of Arabidopsis thaliana Small Secreted Protein Genes
• Effects of Exogenous Proline and Glycinebetaine on the Salt Tolerance of Rice Cultivars
• Display of the human (pro)renin receptor on Bombyx mori nucleopolyhedrovirus (BmNPV) particles using Bm cells(MEDICAL BIOTECHNOLOGY)
• Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H2O2 Accumulation, or Lipid Peroxidation in Arabidopsis thaliana
• Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana

もっと見る 閉じる

スポンサーリンク