Ser84 of Human Renin Contributes to the Biphasic pH Dependence of the Renin-Angiotensinogen Reaction
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概要
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The pH dependence of the reaction of various renins was investigated using sheep angiotensinogen as a substrate. Human renin showed two separate peaks, but rat and mouse Ren1 renins showed one peak with a shoulder. A comparison of the predicted subsite residues of human renin with those of rat and mouse Ren1 renins revealed that Arg82, Ser84, Thr85, Ala229, and Thr312 are unique in the human sequence. We examined the possible importance of these residues in the unique pH profile of the human renin reaction by replacing these residues with the corresponding residues of rat renin. The replacement of Ser84 of human renin with Gly changed the pH dependence of the reaction to one peak, similarly to rat and mouse Ren1 renins. Other mutant human renins kept two separate peaks, similarly to wild-type human renin. These results indicate that Ser84 of human renin contributes to the biphasic pH dependence of the renin-angiotensinogen reaction.
著者
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Suzuki Fumiaki
Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Nakamura Yukio
Laboratory Of Organic Chemistry Faculty Of Technology Kanagawa University
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IWATA Hideyuki
United Graduate School of Agricultural Science, Gifu University
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NAKAGAWA Tsutomu
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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YOSHIOKA Yuichiro
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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NISHIUCHI Kazuhiro
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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SATOU Ryousuke
United Graduate School of Agricultural Science, Gifu University
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HIRATSUKA Tomoaki
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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FUKUI Youko
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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