Ser84 of Human Renin Contributes to the Biphasic pH Dependence of the Renin-Angiotensinogen Reaction
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概要
- 論文の詳細を見る
The pH dependence of the reaction of various renins was investigated using sheep angiotensinogen as a substrate. Human renin showed two separate peaks, but rat and mouse Ren1 renins showed one peak with a shoulder. A comparison of the predicted subsite residues of human renin with those of rat and mouse Ren1 renins revealed that Arg82, Ser84, Thr85, Ala229, and Thr312 are unique in the human sequence. We examined the possible importance of these residues in the unique pH profile of the human renin reaction by replacing these residues with the corresponding residues of rat renin. The replacement of Ser84 of human renin with Gly changed the pH dependence of the reaction to one peak, similarly to rat and mouse Ren1 renins. Other mutant human renins kept two separate peaks, similarly to wild-type human renin. These results indicate that Ser84 of human renin contributes to the biphasic pH dependence of the renin-angiotensinogen reaction.
- 社団法人 日本農芸化学会の論文
- 2007-05-23
著者
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Suzuki Fumiaki
Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University
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Nakamura Yoshimasa
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Takahashi N
Plant Functions Laboratory Riken
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Suzuki F
Laboratory Of Animal Biochemistry Faculty Of Applied Biological Science Gifu University
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Suzuki F
Gifu Univ Gifu Jpn
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Suzuki Fumiaki
Laboratory Of Animal Biochemistry Faculty Of Applied Biological Sciences Gifu University
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Suzuki Fumiaki
Department Of Biotechnology Faculty Of Agriculture Gifu University
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Nakamura Yukio
Laboratory Of Organic Chemistry Faculty Of Technology Kanagawa University
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Suzuki Fumiaki
United Graduate School Of Agricultural Science Gifu University
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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IWATA Hideyuki
United Graduate School of Agricultural Science, Gifu University
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NAKAGAWA Tsutomu
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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YOSHIOKA Yuichiro
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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NISHIUCHI Kazuhiro
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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SATOU Ryousuke
United Graduate School of Agricultural Science, Gifu University
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HIRATSUKA Tomoaki
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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FUKUI Youko
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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Ido Hiroki
United Graduate School Of Agricultural Science Gifu University
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Takahashi Nobuyuki
Hokkaido University Of Education
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Fukui Youko
Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University
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Satou Ryousuke
United Graduate School Of Agricultural Science Gifu University
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Yoshioka Yuichiro
Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University
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Nakano Takeshi
Plant Functions Lab.
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Hiratsuka Tomoaki
Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University
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Nishiuchi Kazuhiro
Laboratory Of Applied Biochemistry Faculty Of Applied Biological Science Gifu University
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NAKAMURA YUKIO
Laboratory of Animal Reproduction, College of Agriculture, University of Osaka Prefecture
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NAKAGAWA Tsutomu
Laboratory of Animal Biochemistry, United Graduate School of Agricultural Science, Gifu University
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