Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
スポンサーリンク
概要
- 論文の詳細を見る
We isolated a novel gene for cyclophilin (CyP) first identified as an intracellular target of the immunosuppressant cyclosporin A and also known to have peptidyl-prolyl cis-trans isomerase(PPIase) activity, named ATCYP5 from Arabidopsis thaliana. ATCYP5 encoded a polypeotide with 201 amino acids with a putative ER-targeting signal sequence at its N-terminal, but without the typical ER-retention signal in its C-terminal. In addition, ATCYP5 protein contained a seven amino-acid long sequence which has been found previously only in cytosolic CyPs from plants. The synthetic mutant green fluorescent protein(sGFP;S65T) was fused to the N-terminal part of ATCYP5, and expressed in tobacco BY-2 cells. The fluorescence derived from the fusion protein was detected mainly around the nucleus, indicating translocation into ER. ATCYP5 was expressed mainly in young stems especially in the apical region and weakly in leaves and roots.
- 日本植物生理学会の論文
著者
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Niwa Yasuo
Plant Science Center Riken(the Institute Of Physical And Chemical Research)
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Niwa Yasuo
Lab. Of Plant Cell Technol. Graduate School Of Nutritional And Environmental Sciences Univ. Of Shizu
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SAITO Takeshi
Research Reactor, Inst., Kyoto Univ.
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Takahashi N
Plant Functions Laboratory Riken
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NAKAGAWA Tsuyoshi
Research Institute of Molecular Genetics, Shimane University
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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MATSUDA Hideyuki
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Tanaka Katsunori
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
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Niwa Yasuo
School Of Food And Nutritional Science University Of Shizuoka
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Ashida Hiroyuki
Research Institute of Molecular Genetics, Shimane University
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Ashida Hiroyuki
Mol. Gen. Res. Inst. Shimane Univ.
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Ashida Hiroyuki
Institute For Molecular Genetics Shimane University
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Takahashi Nobuyuki
Hokkaido University Of Education
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Matsuda H
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Matsuda Hideyuki
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Ashida Hiroyuki
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Saito Takeshi
Research Reactor Institute Kyoto University
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Saito Takeshi
Research Institute of Science and Engineering, Waseda University
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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