Interaction between a Negative Regulator (Msa2/Nrd1) and a Positive Regulator (Cpc2) of Sexual Differentiation in Schizosaccharomyces pombe

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概要

• 論文の詳細を見る

• The sexual differentiation of Schizosaccharomyces pombe is controlled by many cellular components which have not been fully characterized. We isolated a gene called msa2 as a multi-copy suppressor of a sporulation abnormal mutant (sam1). Msa2p is identical with Nrd1p which has been characterized as a factor that blocks the onset of sexual differentiation. The yeast two-hybrid system was used to identify Cpc2p, a fission yeast homolog of the RACK1 protein, that interacted with Msa2p/Nrd1p. We confirmed that Msa2p/Nrd1p interacted with Cpc2p in S. pombe cells. An epistatic analysis of msa2/nrd1 and cpc2 suggests that Msa2p/Nrd1p was an upstream regulator for Cpc2p. A localization analysis of Cpc2p and Msa2p/Nrd1p indicates that both proteins were predominantly localized in the cytoplasm. The interaction of negative regulator Msa2p/Nrd1p with positive regulator Cpc2p suggests a new regulatory circuit in the sexual differentiation of S. pombe.

• 社団法人 日本農芸化学会の論文
• 2004-07-23

著者

• JEONG Hee

  Department of Physics, Pusan National University

• Tanaka K

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• KAWAMUKAI Makoto

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• MATSUDA Hideyuki

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• Tanaka Katsunori

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima

• Tanaka K

  Tottori Univ. Tottori

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• OOWATARI Yasuo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni

• ABE Michiyo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane Uni

• Jeong Hee

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Kawamukai Makoto

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Abe Michiyo

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Oowatari Yasuo

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Morita K

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Kawamukai M

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda H

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda Hideyuki

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Kawamukai Makoto

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Tanaka Keiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kawamukai Makoto

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Koyanagi Takashi

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

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