Identification and Molecular Analysis of Lactococcus lactis rpoD Operon
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概要
- 論文の詳細を見る
The complete nucleotide sequence of an open reading frame (ORF) preceding the Lactococcus lactis rpoD gene is reported. It was suggested that this ORF encodes Lactococcus lactis DNA primase and that the L. lactis rpoD operon consists of only two genes. Northern hybridization analysis showed that i) there are four mRNAs transcribing the rpoD gene (from upstream, M_1-M_4), ii) only the 3. 7-kb transcript M_1 includes the entire rpoD operon, iii) the shortest transcript M_4 exists at both logarithmic and stationary phases of growth while the other three transcripts appear only at logarithmic phase, and iv) no apparent induction at the transcriptional level but transient repression of M_1 was found when the growth temperature was shifted from 30℃ to 42℃. 5'-ends of all four mRNAs were identified by primer extension analyses.
- 社団法人日本農芸化学会の論文
- 1995-01-23
著者
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Shimamura Seiichi
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd.
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ISHIBASHI Norio
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd.
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Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Araya-Kojima Tomoko
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd.
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Tanaka Kan
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Takahashi Hideo
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Araya Tomoko
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd.
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Tanaka Kan
Institute Of Applied Microbiology The University Of Tokyo
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Ishibashi Norio
Nutritional Science Laboratory Morinaga Milk Industry Co. Ltd.
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Shimamura Seiichi
Nutritional Science Laboratory Morinaga Milk Industry Co. Ltd.
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Takahashi H
Tokyo Inst. Technol. Yokohama Jpn
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Araya Tomoko
Nutritional Science Laboratory Morinaga Milk Industry Co. Ltd.
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Takahashi Hideo
Institute Of Applied Microbiology The University Of Tokyo
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TAKAHASHI Hideo
Institue of Applied Microbiology, The University of Tokyo
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