Isolation and Characterization of the Heat-responsive Genes in Escherichia coli
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概要
- 論文の詳細を見る
The ompC gene expression is induced by increasing temperature as well as osmotic pressure. In this study, a mutant (TD2) defective in this thermoresponse was isolated with transposon Tnl0; the mutationi was complemented by pMAN55 or pMAN56 containing micF and mapped at 48min on Escherichia coli K-12. Furthermore, a new gene (hrsA) that suppressed the mutation was cloned. Its nucleotide sequence was analyzed and it was located close to the suc operon at 16.7min corresponding to #18F11 (Kohara bank) on E. coli genome. In TD2 containing the hrsA on a multicopy plasmid, the ompC expression was induced and dependent on OmpR with increased temperature. The HrsA was found to have Enzyme IIA, IIB, and IIC domains that are homologous to Enzyme II; involved in the fructose-specific PTS (phosphotransferase system). The putative phosphorylation sites (His87 and Cys192) were also conserved in HrsA.
- 社団法人日本農芸化学会の論文
- 1996-02-23
著者
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Utsumi Ryutaro
Department of Agricultural Chemistry, Kinki University
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Tanabe Hiroyuki
Department of Agricultural Chemistry, Kinki University
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Takahashi H
Tokyo Inst. Technol. Yokohama Jpn
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Noda Manjiro
Department of Agricultural Chemistry, Kinki University
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Noda Manjiro
Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University
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Utsumi R
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
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Utsumi Ryutaro
Department Of Agricultural Chemistry Kinki University
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Utsumi Ryutaro
Department Of Advanced Bioscience Kinki University
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KATOH Akinori
Department of Agricultural Chemistry, Kinki University
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Katoh Akinori
Department Of Agricultural Chemistry Kinki University
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Horie Tetsuya
Department of Agricultural Chemistry, Kinki University
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KAINO Yoko
Department of Agricultural Chemistry, Kinki University
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Tanabe Hiroyuki
Department Of Agricultural Chemistry Kinki University
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Kaino Yoko
Department Of Agricultural Chemistry Kinki University
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Horie Tetsuya
Department Of Agricultural Chemistry Kinki University
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