A Positive Screening for Drugs that Specifically Inhibit the Ca^<2+>-Signaling Activity on the Basis of the Growth Promoting Effect on a Yeast Mutant with a Peculiar Phenotype
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概要
- 論文の詳細を見る
An inappropriate activation of a signaling pathway in yeast often has a deleterious physiological effect and causes various defects, including growth defects. In a certain genetic background (⊿zds1) of Saccharomyces cerevisiae, the cell-cycle progression in G2 is specifically blocked in the medium with CaCl_2 by the hyperactivation of the Ca^<2+>-signaling pathways. Here, we developed a novel drug screening procedure designed to detect the active compounds that specifically attenuate the Ca^<2+>-signaling activity on the basis of the ability to abrogate the growth defect of the cells suffering from the hyperactivated Ca^<2+> signal. Using known calcineurin inhibitors as model compounds, we have established the screening conditions for the drugs that suppress the Ca^<2+>-induced growth inhibition. An indicator strain with an increased drug sensitivity was constructed with a syr1/erg3 null mutation.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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TAKAHASHI Hidetoshi
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
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HIRATA Dai
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Hirata Dai
Hiroshima Univ. Higashi‐hiroshima Jpn
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Hirata Dai
Department Of Fermentation Technology Hiroshima University
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MIYAKAWA Tokichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Takahashi H
Tokyo Inst. Technol. Yokohama Jpn
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MIZUNUMA Masaki
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter (AdSM), Hirosh
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Mizunuma Masaki
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
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Mizunuma Masaki
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter (adsm) Hiroshim
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SHITAMUKAI Atsunori
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Miyakawa Tokichi
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
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Miyakawa Tokichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Shitamukai Atsunori
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology
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Takahashi Hidetoshi
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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