Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast

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概要

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• Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G1, but the mechanism by which cells restart from G1 delay remains elusive. We found that Whi3, a negative regulator of G1 cyclin, counteracted Hog1 in the restart from G1 delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G1 delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G1 arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G1 arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.

著者

• Hirata Dai

  Department Of Fermentation Technology Hiroshima University

• Ogawa Takafumi

  Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University:instit

• Mizunuma Masaki

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter (adsm) Hiroshim

• Kume Kazunori

  Department Of Molecular Biotechnology Graduate School Of Advanced Science Of Matter Hiroshima Univer

• Shitamukai Atsunori

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• KOYAMA Tetsuya

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• TSUBAKIYAMA Ryohei

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• KOMARUYAMA Tadamasa

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• YAMAGUCHI Toshinaga

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• OGAWA Takafumi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

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