The Physiological Roles of Membrane Ergosterol as Revealed by the Phenotypes of syr1/erg3 Null Mutant of Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Ergosterol is a major sterol component of fungal plasma membranes. The effects of disrupting the Saccharomyces cerevisiae SYR1/ERG3 gene, which encodes sterol C-5 desaturase, an enzyme of ergosterol biosynthesis pathway, were markedly different for different S. cerevisiae strains and growth temperatures. The null mutation of SYR1 (Δsyr1) in strain RAY-3A had only a slight effect on the growth rate at 28℃. However, at this temperature, the same mutation caused poor growth in strain KA-311A and no growth in strain W303-1A. The Δsyrl disruptant of these strains were able to grow at 37℃, as well as their parental strains. Moreover, the growth of the Δsyrl disruptant of W303-1A and KA-311A strains were severely inhibited at 16℃. These results indicated that ergosterol is essential for growth at low temperatures, and the effects of the gene disruption are variable by the genetic background. The growth defect at low temperatures appeared to be due to the defect of tryptophan uptake in the Δsyrl mutants. The Δsyrl mutants were sensitive to a wide variety of drugs, chemicals, and ions, suggesting that yeast ergosterol is important as permeability barrier against various chemical stresses.
- 社団法人日本農芸化学会の論文
- 1995-03-23
著者
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Tsuchiya Eiko
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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HIRATA Dai
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Hirata D
Niigata Prefectural Institute Of Brewing
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Hirata Dai
From The Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Hirata Dai
Center For Gene Science Hiroshima University
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Hirata Dai
Department Of Fermentation Technology Hiroshima University
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MIYAKAWA Tokichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Takemoto Y.
Department of Biology, Utah State University
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Julmanop Charnchai
Department Of Fermentation Technology Hiroshima University
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Tsuchiya E
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
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Tsuchiya Eiko
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Tsuchiya Eiko
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Hirata D
Hiroshima Univ. Higashi‐hiroshima Jpn
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Hemmi Kenji
Department of Fermentation Technology, Hiroshima University
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Hemmi Kenji
Department Of Fermentation Technology Hiroshima University
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Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Miyakawa Tokichi
Department Of Fermentation Technology
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Tsuchiya E
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Takemoto Y.
Department Of Biology And Molecular Biology Program Utah State University
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