A Novel Method of Screening Cell-Cycle Blockers as Candidates for Anti-Tumor Reagents Using Yeast as a Screening Tool
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概要
- 論文の詳細を見る
The mechanisms of eukaryotic cell-cycle regulation are closely linked to cellular tumorigenesis. Compounds that affect the cell cycle are good candidates for developing anti-tumor drugs. We developed a screening method for cell-cycle blockers using a Saccharomyces cerevisiae cdc2-1 rad9Δ strain that can detect the activity of substances by cell growth. We performed screening on culture broth of various microbes, and identified five compounds, borrelidin, mycophenolic acid, UCS15A, copiamycin analog, and fredericamycin A, that were known to possess anti-tumor activity. These results indicate that this screening method is effective as a first-screening system for anti-tumor agents.
著者
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Tsuchiya Eiko
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Yukawa Masashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Ueno Masaru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KIMURA Ken-ichi
Department of Biological Chemistry and Food Science, Iwate University
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TAKAHASHI Hidetoshi
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
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Ueno Masaru
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
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Yukawa Masashi
Dep. Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Univ.
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