Cloning, Characterization, and Expression of cDNA Encoding a Lipase from Kurtzmanomyces sp. I-11(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA clone of the lipase secreted by Kurtzmanomyces sp. I-11 was isolated from a cDNA library of this yeast by PCR screening using oligonucleotide primers designed on the basis of the partial amino acid sequence of the lipase. The cloned cDNA (lip 1) encoded a hydrophobic protein of 484 amino acids, where the first 20 amino acids and the following 6 amino acid sequences were predicted to be the signal sequence for secretion and a pro-sequence, respectively. The deduced amino acid sequence of the Kurtzmanomyces lipase was most similar to Candida antarctica DSM 3855 lipase A (74% identity) and weakly to other lipases. The consensus pentapeptide (-Gly-X-Ser-X-Gly-) that forms a part of the interfacial lipid recognition site in lipases was conserved. A high level of lipase was produced by Pichia pastoris transformed with the lip1 cDNA, indicating that the cloned cDNA indeed encodes a lipase.
- 社団法人日本農芸化学会の論文
- 2002-06-23
著者
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鈴木 修
池浦特許事務所
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SUZUKI Osamu
Department of Fermentation Technology, Hiroshima University
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Kakugawa Koji
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Suzuki O
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Suzuki Osamu
Department Of Cardiology Iwate Prefectural Iwai Hospital
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Suzuki O
Department Of Legal Medicine Hamamatsu University School Of Medicine
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SHOBAYASHI Megumi
Hiroshima Prefectural Institute of Industrial Science and Technology
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MIYAKAWA Tokichi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Sato O
Second Department Of Surgery Faculty Of Medicine The University Of Tokyo
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Suzuki O
Department Of Veterinary Science National Institute Of Infectious Diseases
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Suzuki O
Second Department Of Surgery Yamanashi Medical University
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Miyakawa T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Miyakawa Tokichi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Miyakawa Tokichi
Department Of Fermentation Technology
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Suzuki Osamu
Department Of Veterinary Science National Institute Of Infectious Diseases
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