The Relationship between Sperm Morphology and In Vitro Fertilization Ability in Mice
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概要
- 論文の詳細を見る
In vitro fertilization (IVF) is widely used in reproduction research, but the sperm of some inbred strains of mice yield low fertilization rates in IVF. To determine the cause of this problem, we examined the effect of epididymal sperm morphology, in particular, tail bending and the presence and type of cytoplasmic droplet (CD), on fertilizability in vitro. Sperm suspensions were obtained from the following five strains: C57BL/6J, BALB/cA, C3H/HeN, DBA/2J, and 129x1/SvJ. The sperm were fixed in 10% formalin and three parts of the sperm, namely the head, tail, and CD, were examined. We recorded the proportion of abnormal sperm heads and hairpins at the neck; tails were categorized as straight, proximal bent, or distal bent; and the CDs were categorized as none, light-type, and heavy-type. Based on these parameters, we determined the correlations between sperm morphology and fertilizability in vitro, as judged by IVF using ICR oocytes. The proportion of sperm with a hairpin neck was higher in strain C57BL/6J, while abnormal head morphology occurred significantly more often in strain BALB/cA. The percentage of sperm with a proximal bent tail was highest in strain DBA/2J and lowest in strain 129x1/SvJ. A heavy-type CD was observed more frequently in the 129x1/SvJ and C57BL/6J strains than in the other three strains in which a light-type CD predominated. The rank order of the fertilization rates was 129x1/SvJ < C57BL/6J < C3H/HeN < BALB/cA < DBA/2J. In addition, fertilization rate was positively correlated with a proximal bent tail, but negatively correlated with a heavy-type CD and distal bent tail. This new classification system establishes that the morphological characteristics of epididymal sperm differ among inbred strains of mice and that tail and CD morphology are closely related to fertilization ability in IVF. Thus, our results provide a novel method for assessing the quality of mouse sperm used for IVF.
- 日本繁殖生物学会の論文
- 2006-08-01
著者
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鈴木 修
池浦特許事務所
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HATA Tomoko
Department of Hematology and Molecular Medicine Unit, Atomic Bomb Disease Institute, Nagasaki Univer
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Hata Tomoko
国立環境研究所 獣医科学部
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Hata Tomoko
Department Of Veterinary Science National Institute Of Infectious Diseases
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SUZUKI Osamu
Department of Fermentation Technology, Hiroshima University
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Suzuki O
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Suzuki Osamu
Department Of Cardiology Iwate Prefectural Iwai Hospital
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Suzuki O
Department Of Legal Medicine Hamamatsu University School Of Medicine
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Hata Tomoko
Department Of Hematology And Molecular Medicine Unit Atomic Bomb Disease Institute Nagasaki Universi
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KAWAI Yasuhiro
Department of Pediatrics, Kawasaki Medical School
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Sato O
Second Department Of Surgery Faculty Of Medicine The University Of Tokyo
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Sato Osamu
東京大学 第2外科
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Suzuki O
Department Of Veterinary Science National Institute Of Infectious Diseases
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Suzuki O
Second Department Of Surgery Yamanashi Medical University
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Kawai Yasuhiro
Laboratory Of Experimental Animal Models Division Of Bioresources National Institute Of Biomedical I
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MATSUDA Junichiro
Department of Veterinary Science, National Institute of Infectious Diseases
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Kawai Yasuhiro
Department Of Pediatrics Kawasaki Medical School
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Matsuda Junichiro
Department Of Veterinary Science National Institute Of Infectious Diseases
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Matsuda Junichiro
Department Of Veterinary Science National Institute Of Health
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Suzuki Osamu
Department Of Veterinary Science National Institute Of Infectious Diseases
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