Differentiation and Monitoring of Cells Using a Biochip for Regenerative Medicine : Differentiation of Bone Marrow Stromal Cells and Myoblasts
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概要
- 論文の詳細を見る
A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
- 2006-09-15
著者
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Nakamura Masanori
Department of Clinical Laboratory, and Hyogo Medical Center for Adults
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SUZUKI Osamu
Department of Fermentation Technology, Hiroshima University
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FUKUDA Toshio
Department of Internal Medicine, Shioya General Hospital
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Suzuki Osamu
Department Of Cardiology Iwate Prefectural Iwai Hospital
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ARAI Fumihito
Department of Micro System Engineering, Nagoya University
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Ichikawa Akihiko
National Institute Of Advanced Industrial Science And Technology Intelligent Systems Research Instit
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Katagiri Takenobu
Department Of Biochemistry School Of Dentistry Showa University
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Katagiri Takenobu
Department Of Dentistry Saitama Medical School
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Arai Fumihito
Department Of Bioengineering And Robotics Tohoku University
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Fukuda Toshio
Department Of Micro-nano Systems Engineering Nagoya University
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KAMIJO Ryutaro
Department of Biochemistry, Showa University School of Dentistry
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UCHIDA Tomoyuki
Department of Micro-Nano Systems Engineering, Nagoya University
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NUMATA Mamoru
JGC Corporation
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WATANABE Naruaki
JGC Corporation
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Kamijo Ryutaro
Department Of Biochemistry Showa University School Of Dentistry
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Kamijo Ryutaro
Department Of Dentistry Showa University
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Nakamura Masanori
Department Of Dentistry Showa University
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Nakamura Masanori
Department Of Clinical Laboratory And Hyogo Medical Center For Adults
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Uchida Tomoyuki
Department Of Information Systems Kyushu University
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Uchida Tomoyuki
Department Of Micro-nano Systems Engineering Nagoya University
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Suzuki Osamu
Department Of Dentistry Tohoku University
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Fukuda Toshio
Department Of Cytopathology Graduate School Of Health Sciences Gunma University
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Arai Fumihito
Department of Bioengineering and Robotics, Tohoku University
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NAKAMURA Masanori
Department of Applied Chemistry, Faculty of Engineering, Toyo University
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