In Vitro Replication of Nuclear Deoxyribonucleic Acid in Isolated Nuclei of Saccharomyces cerevisiae

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概要

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• The incorporation reaction of [3H-methyl]deoxyribothymidine triphosphate (3H-dTTP) into the TCA-insoluble fraction by isolated nuclei of Saccharomyces cerevisiae was characterized as follows : (i) addition of three deoxyribonucleotides (dATP, dGTP and dCTP) is required for maximal incorporation, (ii) the buoyant density of the incorporated products is approximately 1.70, (iii) the incorporation reaction is strongly inhibited by inhibitors of DNA synthesis, (iv) 98% of radio-activities in the products are recovered in the DNA fraction, (v) the products are DNasesensitive and appear as long fibers at a high frequency, and (vi) the increase in accumulation of small-fragment DNA is time-dependent during the first 3-min incubation, followed by a rapid increase in large-fragment DNAs.Thus, it was concluded that the isolated yeast nuclei have an ability to synthesize or replicate nuclear DNA in vitro. Theability of isolated nuclei was strongly stimulated by cytoplasmic factor(s) in the cellular-soluble fraction prepared from mid-log cells of S. cerevisiae.

• 社団法人 日本農芸化学会の論文

著者

• Kamimura Kazuo

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• Fukui Sakuzo

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Tsuchiya Eiko

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Miyakawa Tokichi

  Department Of Fermentation Technology

• TSUCHIYA Eiko

  Department of Fermentation Technology, Faculty of Engineering, University of Hiroshima

• FUKUI Sakuzo

  Department of Fermentation Technology, Faculty of Engineering, University of Hiroshima

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