Purification and Characterization of Sulfide : Quinone Oxidoreductase from an Acidophilic Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans

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概要

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• Sulfide:quinone oxidoreductase (SQR) was purified from membrane of acidophilic chemolithotrophic bacterium Acidithiobacillus ferrooxidans NASF-1 cells grown on sulfur medium. It was composed of a single polypeptide with an apparent molecular mass of 47 kDa. The apparent Km values for sulfide and ubiquinone were 42 and 14 μM respectively. The apparent optimum pH for the SQR activity was about 7.0. A gene encoding a putative SQR of A. ferrooxidans NASF-1 was cloned and sequenced. The gene was expressed in Escherichia coli as a thioredoxin-fusion protein in inclusion bodies in an inactive form. A polyclonal antibody prepared against the recombinant protein reacted immunologically with the purified SQR. Western blotting analysis using the antibody revealed an increased level of SQR synthesis in sulfur-grown A. ferrooxidans NASF-1 cells, implying the involvement of SQR in elemental sulfur oxidation in sulfur-grown A. ferrooxidans NASF-1 cells.

• 社団法人 日本農芸化学会の論文
• 2007-11-23

著者

• Kikumoto Mei

  Tajimi City Pottery Design and Technical Center

• Kanao Tadayoshi

  Graduate School of Natural Science and Technology, Okayama University

• Wakai Satoshi

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Kanao Tadayoshi

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Kamimura Kazuo

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Kamimura K

  Graduate School Of Natural Science And Technology Okayama University

• Kamimura Kazuo

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• TSUJITA Mizuho

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• KIKUMOTO Mei

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• MANCHUR Mohammed

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Manchur Mohammed

  Graduate School Of Natural Science And Technology Okayama University:(present Office)department Of M

• Kanao T

  Graduate School Of Natural Science And Technology Okayama University

• Kanao Tadayoshi

  Faculty Of Agriculture Okayama University

• Kanao Tadayoshi

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• Kamimura Kazuo

  Department Of Botany And Microbiology Division Of Bioscience Graduate School Of Natural Science And

• Tsujita Mizuho

  Department Of Botany And Microbiology Division Of Bioscience Graduate School Of Natural Science And

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