Isolation and Some Properties of Thiobacillus ferrooxidans Strains with Differing Levels of Mercury Resistance from Natural Environments

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概要

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• Fifty iron-oxidizing bacteria isolated from natural environments were screened for resistance to mercuric ions (Hg^<2+>). Thiobacillus ferrooxidans Funis 2-1, the strain found to show the greatest resistance to Hg^<2+> among the fifty isolates, gave a cell yield of 7.0×10^7 cells/ml after 8 d cultivation in an Fe^<2+>-medium (pH 2.5) containing 0.7μM Hg^<2+>. Funis 2-1 volatilized 80% of the total mercury added to the medium over 8 d of cultivation. T.ferrooxidans AP19-3, more sensitive to Hg^<2+> than Funis 2-1, could not grow in an Fe^<2+>-medium (pH 2.5) containing 0.7 μM Hg^<2+> even over a 28 d cultivation period. When resting cells of strains Funis 2-1 and AP 19-3 were incubated for 3 h in a salt solution containing 0.7 μM Hg^<2+> (pH 3.0), 14.3% and 7.9% of the total mercury added to the reaction mixtures respectively, were volatilized. The activity of the mercuric reductase from Funis 2-1 was only 2.8 times higher than that of the enzyme from AP19-3. Since the markedly higher mercury resistance of Funis 2-1 compared with that of AP19-3 cannot be explained only by the level of the mercuric reductase activity, the levels of mercury resistance of iron oxidase and cytochrome c oxidase were studied. The 1 μM mercuric ions inhibited the 35% of iron-oxidizing activity from A19-3. In contrast, the same concentration of Hg^<2+> did not inhibit the activity of iron oxidase from Funis 2-1. In the case of the cytochrome c oxidases purified from both strains, the 0.2 μM Hg^<2+> inhibited approximately 40% of cytochrome c oxidizing activity from AP19-3, on the contrary, the activity of the enzyme from Funis 2-1 was activated 1.8- and 1.2- fold, respectively, in the presence of 0.08 and 0.2 μM Hg^<2+>. Since cytochrome c oxidase is one of the most important components of the iron-oxidizing system, these results indicate that both the existence of cytochrome c oxidase resistant to Hg^<2+> as well as that of mercuric reductase in the cells is responsible for the more rapid growth of Funis 2-1 than that of in an Fe^<2+>-medium containing 0.7 μM Hg^<2+>.

• 社団法人日本生物工学会の論文
• 1999-10-25

著者

• TAKEUCHI Fumiaki

  Environmental Management Center, Okayama University

• Kamimura Kazuo

  Graduate School of Natural Science and Technology, Okayama University

• TAKEUCHI FUMIAKI

  Administration Center for Environmental Science and Technology, Okayama University

• Sugio Tsuyoshi

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Kamimura Kazuo

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• IWAHORI KENJI

  Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni

• Sugio Tsuyoshi

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• Sugio Tsuyoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University

• Sugio Tsuyoshi

  Department Of Biological Function And Genetic Resources Science Okayama University

• Iwahori K

  Okayama Univ. Okayama Jpn

• Iwahori Kenji

  Division Of Science And Technology For Energy Conversion Graduate School Of Natural Science And Tech

• Kamimura K

  Graduate School Of Natural Science And Technology Okayama University

• Kamimura Kazuo

  Marine Biological Technology Section Chugoku National Industrial Research Institute

• Kamimura Kazuo

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• 杉尾 剛

  Div. Of Bioscience Graduate School Of Natural Sci. And Technol. Okayama Univ.

• Takeuchi Fumiaki

  Administration Center For Environmental Science And Technology Okayama University

• Takeuchi F

  Environmental Management And Safety Section Health And Environment Center Okayama Univ.

• Takeuchi Fumiaki

  Environmental Management Center Okayama Univ.

• Sugio T

  Okayama Univ. Okayama Jpn

• Kamimura Kazuo

  Department Of Botany And Microbiology Division Of Bioscience Graduate School Of Natural Science And

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