Isolation and Some Properties of an Iron-oxidizing Bacterium Thiobacillus ferrooxidans Resistant to Molybdenum Ion
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概要
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Among seventy five strains of iron-oxidizing bacteria obtained from natural environments, only one strain, Thiobacillus ferrooxidans Funis 2-1, grew on Fe^<2+>-medium with 1.25mM of sodium molybdate (Mo^<6+>). In contrast, T. ferrooxidans AP19-3, the representative of Mo sensitive strains, could not grow on Fe^<2+> -medium with 1.0 mM of sodium molybdate. By comparing the levels of inhibition of iron oxidase and cytochrome c oxidase by Mo^<6+> or Mo^<5+>, it was found that Mo^<5+> but not Mo^<6+> is an actual inhibitor for the iron oxidation enzyme system, especially for cytochrome c oxidase. Cytochrome c oxidase of Funis 2-1 was more resistant to Mo^<5+> than AP19-3. Mo^<5+>, compared to Mo^<6+>, strongly binds to both cells and the plasma membrane of T. ferrooxidans. Funis 2-1 cells showed a lower binding activity to Mo^<6+> or Mo^<5+> compared to AP19-3. Cytochrome c oxidase of T. ferrooxidans has been known to catalyze the oxidation of not only reduced mammalian cytochrome c but also Mo^<5+>. Mo^<6+> -oxidizing activities medsured with intact cells and a purified cytochrome c oxidase from Funis 2-1 cells were higher than those of AP19-3, suggesting that Funis 2-1 cells can oxidize toxic Mo^<5+> more rapidly to harmless Mo^<6+> than AP19-3 does. Since Mo^<6+> is known to be chemically reduced by Fe^<2+> to give Mo^<5+> and Fe^<3+>, the growth inhibition by sodium molybdate(Mo^<6+>) observed in T. ferrooxidans is explained as follows: Mo^<6+> added to Fe^<6+> -medium is chemically reduced by Fe^<2+>, and Mo^<5+> thus produced binds to the plasma membrane and inhibits iron oxidase, as a result, growth of the bacterium is stopped.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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OSHIMA Mitsuko
Department of Chemistry, Faculty of Science, Okayama University
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BLAKE II
Xavier University of Louisiana
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Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Yong Ng
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Oshima Mitsuko
Department Of Analytical Chemistry Faculty Of Science Okayama University
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Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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Blake Ii
Xavier University
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Sugio Tsuyoshi
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Blake 2
Xavier University
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