Solubilization of Cu^<2+> from Copper Ore by Iron-Oxidizing Bacteria Isolated from the Natural Environment and Identification of the Enzyme That Determines Cu^<2+> Solubilization Activity
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概要
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Solubilization of Cu^<2+> from copper concentrate by 67 strains of iron-oxidizing bacteria isolated from the natural environment was studied. In the case of static growth on copper concentrate (5%)-salt medium (pH 2.5) at 30℃, Cu^<2+> solubilization activities of 65 starins including Thiobacillus ferrooxidans strains AP19-3,OK-2,OK-3,OK1-50,Funis and NASF-1 ranged from 2.5-3.5 mg Cu^<2+>/ml/33 d. In contrast, the activities of T. ferrooxidans strains KO-1 and NA-1 were quite low at 0.9 mg Cu^<2+>/ml/33 d. Nonbiological solubilization activity of Cu^<2+> was 0.4 mg Cu^<2+>/ml/33 d. For identification of the enzymes that play a crucial role in biological Cu^<2+> solubilization, the activities of the enzymes involved in iron and sulfur oxidations were compared among the strains described above. No significant differences in the growth on iron-salt medium and the levels of cellular iron oxidizing activity were observed. In constant, a marked difference was observed in the cellular activity of an enzyme that was involved in the first step of sulfur oxidation in T. ferrooxidans, i.e., hydrogen sulfide : ferric ion oxidoreductase (SFORase). Strains Funis, OK1-50,NASF-1,OK-3,AP19-3,OK-2 which showed high Cu^<2+> solubilization activity showed high SFORase activity (1.3-4.0 μmol Fe^<2+>/ml/4 h). In contrast, strains NA-1 and KO-1 which showed low Cu^<2+> solubilization activity showed low SFORase activity (0.3-0.4 μmol Fe^<2+>/ml/4h), suggesting that SFORase, but not iron oxidase, is the enzyme that determines the ability of T. ferrooxidans to solubilize Cu^<2+> from copper concentrate.
- 社団法人日本生物工学会の論文
- 1996-10-25
著者
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Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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AKHTER FAUZIA
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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Akhter Fauzia
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Sugio Tsuyoshi
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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