Isolation and Some Properties of a Strain of the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans Resistant to 2, 4-Dinitrophenol

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Among two hundred strains of iron-oxidizing bacteria isolated from the natural environment, only one, Thiobacillus ferrooxidans NASF-1, grew in an Fe^<2+> medium supplemented with 10 μM of 2, 4-dinitrophenol(2, 4-DNP). In contrast, growth of T.ferrooxidans AP19-3, a strain representative of iron-oxidizing bacteria sensitive to 2, 4-DNP, was strongly inhibited by 2, 4-DNP at 1.0 μM, and completely repressed at 10 μM. Strain NASF-1 was also resistant to an other uncoupler, carbonylcyanide m-chlorophenylhydrazone(CCCP). The iron-oxidizing activities of strains AP19-3 and NASF-1 were respectively inhibited 69 and 23% by 10 μM 2, 4-DNP, while the ^<14>CO_2 uptake activities of the two strains were inhibited 31 and 5% by 2, 4-DNP at 1.0 μM, and 100 and 89% at 5.0 μM. When intact cells of strain AP19-3 were incubated with Fe^<2+> and 1.0 μM 2, 4-DNP for 10 min at pH 3.0, 49 pmol 2, 4-DNP per mg of protein was incorporated into the cells. In contrast, strain NASF-1 did not incorporate 2, 4-DNP under the same conditions, suggesting that, compared with strain NASF-1, strain AP19-3 has a plasma membrane through which 2, 4-DNP is more easily incorporated in the cytosol.
社団法人日本生物工学会の論文
1998-07-25