Existence of Two Kinds of Sulfur-reducing Systems in Iron-oxidizing Bacterium Thiobacillus ferrooxidans
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概要
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Intact cells of Thiobacillus ferrooxidans NASF-1 incubated under anaerobic conditions in a reaction mixture containing 0.5% colloidal sulfur produced hydrogen sulfide (H_2S) extracellularly. The amount of H_2S produced by cells increased corresponding to the cell amounts and colloidal sulfur. Two activity peaks of H_2S production were observed at pH 1.5 and 7.5. We tentatively called the enzyme activities pH 1.5-and pH 7.5-sulfur reducing systems, respectively. Seven strains of T. ferrooxidans tested had both the activities of pH 1.5-and pH 7.5-sulfur reducing systems, but at different levels. T. ferrooxidans NASF-1 showed the highest activity of the pH 1.5-sulfur reducing system and strain 13598 from ATCC showed the highest activity of the pH 7.5-sulfur reducing system. Further characteristics of H_2S production were studied with intact cells of NASF-1. The optimum temperatures for pH 1.5-and pH 7.5-sulfur reducing systems of NASF-1 were 40℃. Hydrogen sulfide production continued for 8 days and total amounts of H_2S produced at pH 7.5 and 1.5 were 832 and 620 nmol/mg protein, respectively. The pH 7.5-sulfur reducing system used only colloidal sulfur as the electron acceptor. However, the pH 1.5-sulfur reducing system used both colloidal sulfur and tetrathionate. Thiosulfate, dithionate, and sulfite could not be used as the electron acceptor for both of the sulfur reducing systems. Potassium cyanide activated by 3-fold the pH 1.5-sulfur reducing system activity at 0.5 mM but did not affect the activity of the pH 7.5-sulfur reducing system. An inhibitor of sulfite reductase, p-chloromercuribenzene sulfonic acid, did not affect either enzyme activity. Sodium molybdate and monoiodoacetic acid strongly inhibited the activity of the pH 1.5-sulfur reducing system at 1.0 mM, but not the activity of pH 7.5-sulfur reducing system.
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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Kamimura Kazuo
Graduate School of Natural Science and Technology, Okayama University
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Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Kamimura Kazuo
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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INOUE SAYURI
Department of Biological Function, Faculty of Agriculture, Okayama University
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NG KimYong
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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FUJIOKA Akira
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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Ng K
Okayama Univ. Okayama Jpn
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Ng Kim
Division Of Science And Technology For Energy Conversion Graduate School Of Natural Science And Tech
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Inoue S
Okayama Univ. Okayama
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Fujioka Akira
Department Of Dermatology National Defence Medical College
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Fujioka Akira
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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Sugio Tsuyoshi
Department Of Biological Function And Genetic Resources Science Okayama University
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Kamimura K
Graduate School Of Natural Science And Technology Okayama University
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Kamimura Kazuo
Marine Biological Technology Section Chugoku National Industrial Research Institute
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Kamimura Kazuo
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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杉尾 剛
Div. Of Bioscience Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Inoue Sayuri
Department Of Biological Function Faculty Of Agriculture Okayama University
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Inoue S
Tochigi Research Laboratories Of Kao Corporation
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Sugio T
Okayama Univ. Okayama Jpn
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Kamimura Kazuo
Department Of Botany And Microbiology Division Of Bioscience Graduate School Of Natural Science And
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Ng Kim
Department Of Mechanical Engineering Faculty Of Engineering National University Of Singapore
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