Subunit Structure of Glucoamylase of Saccharomyces diastaticus
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概要
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Extracellular glucoamylase produced by a starch-fermenting yeast, Saccharomyces diastaticus 5106-9A, was purified. The enzyme was found to be heterogeneous in molecular weight, ranging from approximately 80K to 66K as estimated by gel filtration, and consisted of two subunits, H and Y. The molecular weight of subunit Hwas heterogeneous and was determined to be approximately 68K, 59K, and 53K by acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The molecular weight of subunit Y was 14K, estimated by the same gel. the molecular weight of the deglycosylated form of subunit H was 4IK, suggesting that the heterogeneity of the enzyme was due to glycosyl moieties of subunit H. Subunits H and Y were separated by gel filtration in the presence of sodium dodecyl sulfate. Subunit Y seemed to be hydrophobic, since it was insoluble in an aqueous buffer without detergent.
- 社団法人 日本農芸化学会の論文
著者
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Fukui Sakuzo
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Hatano Takushi
Department Of Bioengineering Faculty Of Engineering Fukuyama University
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Yamashita Ichiro
Department Of Electronics Faculty Of Engineering Kyoto University
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Hatano Takushi
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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