Expression of the Carboxymethylcellulase Gene, CMCl, from Cryptococus flavus in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• A cDNA copy of the carboxymethylcellulase (CMCase 1) gene, CMC1,of the yeast Cryprococcus flavus was expressed in Saccharomyces cerevisiae under the control of the glycereldehyde-3-phosphate dehydrogenase gene (GAP) promotor derived from S. cerevisiae. In glucose medium, extracellular production of a large amount of CMCase 1 was achieved by S. cerevisiae cells transformed with YEp-plasmid carrying CMC1. Two major proteins (36 kDa and 34 kDa) secreted in the medium both and had CMCase activity and immuno-responsibility to anti-CMCase 1-antibody. From an analysis of amino acid sequences, it was round that the 36 kDa protein is pro-CMCase 1 having an N-terminal residue, Ala, the 19th amino acid od the peptide deduced from the nucleotide sequence of CMC1 on the cDNA, and that the 34 kDa protein is a mature CMCase 1. Alteration of the cell morphology of S. cerevisiae was induced by trabsformation with YEp-placmid carrying CMC1.

• 社団法人日本生物工学会の論文
• 1994-03-25

著者

• HATANO TAKUSHI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• FUKUI SAKUZO

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• MIYAKAWA Tokichi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• MATSUZAKI HIROAKI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• Mochizuki Daisuke

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Matsuzaki Hiroaki

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Fukui Sakuzo

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• Hatano Takushi

  Department Of Bioengineering Faculty Of Engineering Fukuyama University

• HITANO TAKUSHI

  Department of Bioengineering, Faculty of Engineering, Fukuyama University

• KOMURA TSUNEHIRO

  Department od Bioengineering, Faculty of Engineering, Fukuyama University

• CUI ZHIFENG

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Cui Zhifeng

  Department Of Molecular Biotechnology Faculty Of Engineering Hiroshima University

• Miyakawa T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Miyakawa Tokichi

  Department Of Fermentation Technology

• Komura Tsunehiro

  Department Od Bioengineering Faculty Of Engineering Fukuyama University

• CUI Zhifeng

  Department of Bio-Geosciences, Osaka City University

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