Studies of Cosmid Transduction in Streptomyces lividans and Streptomyces parvulus(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
Cosmid pR4Cl is a derivative of multicopy plasmid pIJ365 which has an insertion of the cos (cohesive end site) region of actinophage R4 [T. Morino, H. Takahashi and H. Saito, Mol. Gen. Genet., 198, 228 (1985)]. When the donor R4 phage was propagated in S. lividans carrying the plasmid, the phage lysate contained transducing particles which encapsulated head-to-tail concatemers of the plasmid DNA. These particles could mediate transfer of the plasmid at a high frequency. We examined conditions that gave a maximum transduction frequency of cosmid pR4Cl. Conditions which depress R4 phage propagation, such as incubation of recipient S. parvulus at a high temperature, improved the frequency. Obviously such conditions minimized the lethal effect of viable phage propogation. The highest transduction frequency obtained so far was around 3 × 10^<-3> transductants per infected phage when S. lividans was used as the recipient. This was about 30 per cent of the cosmid transducing particles estimated from the cosmid DNA content in the transducing lysate. The significance of cosmid transduction for gene manipulation in Streptomyces strains is also discussed.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
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Tomita Koji
Bristol-myers Squibb Research Institute
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Takahashi Hideo
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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NAKAMURA Tsuneo
Research Institute of Electronics, Shizuoka University
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Takahashi H
Tokyo Inst. Technol. Yokohama Jpn
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Saito H
National Research Inst. Fisheries Science Ministry Of Agriculture Forestry And Fisheries Tokyo Jpn
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TAKAGI Kenkichi
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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MORINO Tomio
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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TAKITA Tomohisa
Research Laboratory, Nippon Kayaku Co., Ltd.
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SAITO Hiuga
Research Laboratory, Nippon Kayaku Co., Ltd.
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Morino T
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Morino Tomio
Research And Development Division Pharmaceutical Group Nippon Kayaku Co. Ltd.
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Tomita Katsuhisa
Research Institute Fancl Co.
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Takita T
Research Laboratory Nippon Kayaku Co. Ltd.
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Takita Tomohisa
Research Laboratories Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Nakamura Tsuneo
Research Laboratory Nippon Kayaku Co. Ltd.
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Nakamura Tsuneo
Research Institute Of Electronics Shizuoka University:(present Address)sharp Co. Lid.
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Takahashi Hideo
Institute Of Applied Microbiology The University Of Tokyo
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TAKAHASHI Hideo
Institue of Applied Microbiology, The University of Tokyo
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