Isolation and Characterization of the groES and groEL genes of Bacillus subtilis Marburg

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概要

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• The complete set of groES and groEL gene homologues from Bacillus subtilis Marburg 168 was identified, cloned, and characterized. The nucleotide sequence indicated the presence of two open reading frames corresponding to the groES and groEL genes. The presumptive GroES and GroEL proteins were calculated to be polypeptides of 10,175 and 57,175Da, respectively, and showed extensive sequence similarities with the known GroES and GroEL proteins of Escherichia coli and Mycobacterium tuberculosis. A heat-inducibel transcript initiated upstream of the groES coding region was identified by primer-extension analysis of in vivo transcripts, indicating that the two genes consist of an operon. At least six heat-shock inducible proteins were identified in the cell extract of heat treated B.subtilis. Two proteins of 10 and 60 kDa overproduced in B.subtilis cells carrying a multi-copy groES and groEL plasmid were demonstrated to correspond to two out of the six heat-shock inducible proteins. The groES and groEL genes of B.subtilis were physically mapped on the 60° region of a 360°map and genetically mapped at the position of 40% linkage with the purB locus using PBS1 transduction of groEl genes tagged with a chloramphenicol resistance (chl') marker.

• 社団法人日本農芸化学会の論文
• 1992-12-23

著者

• Tozawa Y

  Mitsubishi Kagaku Inst. Of Life Sci. Yokohama Jpn

• Takahashi Hideo

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• Takahashi H

  Institue Of Molecular And Cellular Biosciences The University Of Tokyo : Department Of Applied Biolo

• Kawamura Fujio

  Institute Of Applied Microbiology The University Of Tokyo

• Kawamura Fujio

  Institue Of Applied Microbiology The University Of Tokyo

• TOZAWA Yuzuru

  Institue of Applied Microbiology, The University of Tokyo

• YOSHIKAWA Hirofumi

  Institue of Applied Microbiology, The University of Tokyo

• ITAYA Mitsuhiro

  Mitsubishi Kasei Institute of Life Sciences

• Tozawa Yuzuru

  Institue Of Applied Microbiology The University Of Tokyo

• Yoshikawa H

  Tokyo Univ. Agriculture Tokyo Jpn

• Itaya Mitsuhiro

  Mitsubishi Kagaku Institute Of Life Sciences

• Takahashi Hideo

  Institute Of Applied Microbiology The University Of Tokyo

• Itaya Mitsuhiro

  Mitsubishi Kagaku Inst. Of Life Sciences

• TAKAHASHI Hideo

  Institue of Applied Microbiology, The University of Tokyo

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