Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome : an Efficient Selection Method
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概要
- 論文の詳細を見る
The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
- 社団法人 日本農芸化学会の論文
- 2005-05-23
著者
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Tsuge Kenji
Mitsubishi Kagaku Institute Of Life Sciences
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ITAYA Mitsuhiro
Mitsubishi Kasei Institute of Life Sciences
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Uotsu Tomita
Mitsubishi Kagaku Institute Of Life Sciences
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KANEKO Shinya
Mitsubishi Kagaku Institute of Life Sciences
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Itaya Mitsuhiro
Mitsubishi Kagaku Institute Of Life Sciences
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Kaneko Shinya
Mitsubishi Kagaku Inst. Life Sci. Tokyo
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Itaya Mitsuhiro
Mitsubishi Kagaku Inst. Of Life Sciences
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Uotsu-Tomita Rie
Mitsubishi Kagaku Institute of Life Sciences
関連論文
- Isolation and Characterization of the groES and groEL genes of Bacillus subtilis Marburg
- Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector
- Efficient Cloning and Engineering of Giant DNAs in a Novel Bacillus subtilis Genome Vector
- Reshuffling of the Bacillus subtilis 168 Genome by Multifold Inversion
- Green Marker for Colonies of Bacillus subtilis
- Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome : an Efficient Selection Method
- Region Dependent Efficiency for Recombinational Transfer of the Bacillus subtilis 168 Genome
- A Method to Invert DNA Segments of the Bacillus subtilis 168 Genome by Recombination between Two Homologous Sequences
- Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium
- Experimental Basis for a Stable Plasmid, pLS30, to Shuttle between Bacillus subtilis Species by Conjugational Transfer
- Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome
- Conversion of Bacillus subtilis 168 : Natto Producing Bacillus subtilis with Mosaic Genomes
- First Evidence for Homologous Recombination-mediated Large DNA Inversion on the Bacillus subtilis 168 Chromosome
- Construction of a Novel Tetracycline Resistance Gene Cassette Useful as a Marker on the Bacillus subtilis Chromosome
- Novel Exopolygalacturonases Produced by Alternaria mali
- Designed horizontal transfer of stable giant DNA released from Escherichia coli
- Sequential Insertion of Multiple I-SceI Recognition Sites at Designed Loci of the Bacillus subtilis 168 Genome
- Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector