Green Marker for Colonies of Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.
- 社団法人日本農芸化学会の論文
- 2001-03-23
著者
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Shaheduzzaman Syed
Mitsubishi Kasei Institute Of Life Sciences
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Omori Akira
Mitsubishi Kagaku Institute Of Life Science (mitils)
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Omori A
Mitsubishi Kasei Institute Of Life Sciences
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ITAYA Mitsuhiro
Mitsubishi Kasei Institute of Life Sciences
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TSUJI Takashi
Mitsubishi Kasei Institute of Life Sciences
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MATSUI Kuniko
Mitsubishi Kagaku Institute of Life Sciences
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Matsui Kuniko
Mitsubishi Kasei Institute Of Life Sciences
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Itaya Mitsuhiro
Mitsubishi Kagaku Institute Of Life Sciences
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Itaya Mitsuhiro
Mitsubishi Kasei Institute Of Life Sciences:(present Address) Nci National Institutes Of Healt
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Itaya Mitsuhiro
Mitsubishi Kagaku Inst. Of Life Sciences
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