Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome
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概要
- 論文の詳細を見る
The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomvcin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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Itaya Mitsuhiro
Mitsubishi Kagaku Institute Of Life Sciences
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Itaya Mitsuhiro
Mitsubishi Kagaku Inst. Of Life Sciences
関連論文
- Isolation and Characterization of the groES and groEL genes of Bacillus subtilis Marburg
- Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector
- Efficient Cloning and Engineering of Giant DNAs in a Novel Bacillus subtilis Genome Vector
- Green Marker for Colonies of Bacillus subtilis
- Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome : an Efficient Selection Method
- Region Dependent Efficiency for Recombinational Transfer of the Bacillus subtilis 168 Genome
- A Method to Invert DNA Segments of the Bacillus subtilis 168 Genome by Recombination between Two Homologous Sequences
- Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium
- Experimental Basis for a Stable Plasmid, pLS30, to Shuttle between Bacillus subtilis Species by Conjugational Transfer
- Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome
- Conversion of Bacillus subtilis 168 : Natto Producing Bacillus subtilis with Mosaic Genomes
- First Evidence for Homologous Recombination-mediated Large DNA Inversion on the Bacillus subtilis 168 Chromosome
- Construction of a Novel Tetracycline Resistance Gene Cassette Useful as a Marker on the Bacillus subtilis Chromosome
- Novel Exopolygalacturonases Produced by Alternaria mali
- Designed horizontal transfer of stable giant DNA released from Escherichia coli
- Sequential Insertion of Multiple I-SceI Recognition Sites at Designed Loci of the Bacillus subtilis 168 Genome
- Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector