A Method to Invert DNA Segments of the Bacillus subtilis 168 Genome by Recombination between Two Homologous Sequences

スポンサーリンク

概要

• 論文の詳細を見る

• We developed a method that allows rapid isolation of mutant Bacillus subtilis 168 carrying an inversion of a specific DNA segment of the genome. Two incomplete neomycin resistance gene cassettes were integrated at both ends of the 1652-kb segment to be inverted. Reciprocal recombination within the 590-bp homologous region of these two cassettes created an intact neomycin resistance gene with concomitant inversion of the 1652-kb segment flanked by the two cassettes. Structure of the mutant genome was verified by analyzing the physical map for rare cutting endonucleases, SfiI, NotI, I-CeuI, and I-SceI. The inversion rate was estimated to be 6.9±1.4×10^<-8>/cell/cell division at 37℃. The method should be in principle applicable not only to other regions of the B. subtilis genome but also to other bacterial genomes.

• 社団法人日本農芸化学会の論文
• 1996-05-23

著者

• TODA Tsutomu

  Mitsubishi Kagaku Institute of Life Sciences

• Tanaka Teruo

  School Of Marine Science And Technology Tokai University

• Itaya Mitsuhiro

  Mitsubishi Kagaku Institute Of Life Sciences

• Toda Tsutomu

  Mitsubishi Kasei Institute Of Life Sciences

• Itaya Mitsuhiro

  Mitsubishi Kagaku Inst. Of Life Sciences

関連論文

• Inactivation of Blasticidin S by Bacillus cereus. VI. Structure and Comparison of the bsr Gene from a Blasticidin S-Resistant Bacillus cereus
• Nucleotide Sequence of the bsr Gene and N-Terminal Amino Acid Sequence of Blasticidin S Deaminase from Blasticidin S Resistant Eshcerichia coli TK121(Biological Chemistry)
• Isolation and Characterization of the groES and groEL genes of Bacillus subtilis Marburg
• Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector
• Efficient Cloning and Engineering of Giant DNAs in a Novel Bacillus subtilis Genome Vector
• Reshuffling of the Bacillus subtilis 168 Genome by Multifold Inversion
• Green Marker for Colonies of Bacillus subtilis
• Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome : an Efficient Selection Method
• Region Dependent Efficiency for Recombinational Transfer of the Bacillus subtilis 168 Genome
• A Method to Invert DNA Segments of the Bacillus subtilis 168 Genome by Recombination between Two Homologous Sequences
• Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium
• Experimental Basis for a Stable Plasmid, pLS30, to Shuttle between Bacillus subtilis Species by Conjugational Transfer
• Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome
• Conversion of Bacillus subtilis 168 : Natto Producing Bacillus subtilis with Mosaic Genomes
• First Evidence for Homologous Recombination-mediated Large DNA Inversion on the Bacillus subtilis 168 Chromosome
• Construction of a Novel Tetracycline Resistance Gene Cassette Useful as a Marker on the Bacillus subtilis Chromosome
• Novel Exopolygalacturonases Produced by Alternaria mali
• Designed horizontal transfer of stable giant DNA released from Escherichia coli
• Sequential Insertion of Multiple I-SceI Recognition Sites at Designed Loci of the Bacillus subtilis 168 Genome
• Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector

もっと見る 閉じる

スポンサーリンク