Construction of Phage Vectors in Streptomyces: Introduction of the Thiostreptone Resistant (tsr) Gene into R4 Phage
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概要
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We have constructed phage cloning vectors from an actinophage, R4. A deletion derivative (R4 22B) which had a BamHllinker inserted at the unique PvuII site was used to clone the thiostreptone resistant (tsr) gene derived from plasmid vector pIJ365. The tsr derivative obtained, R422B-tsr1, was shown to have the same level of thiostreptone resistance in lysogenized cells as that of pIJ365-carrying cells. Under the optimal conditions, R422B-tsrl phage was lysogenized at a frequency of 5×10-2 per infected phage. The usefulness of R4 phage derivatives for gene cloning is discussed.
- 社団法人 日本農芸化学会の論文
著者
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Saito Hiuga
Institute Of Applied Microbiology University Of Tokyo
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Takahashi Hideo
Institute Of Applied Microbiology The University Of Tokyo
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ISOGAI Takao
Institute of Applied Microbiology, The University of Tokyo
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MORINO Tomio
Institute of Applied Microbiology, The University of Tokyo
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