Cloning of Oxetanocin A Biosynthetic and Resistance Genes that Reside on a Plasmid of Bacillus megaterium Strain NK84-0128
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概要
- 論文の詳細を見る
Bacillus megaterium strain NK84-0218 produces a potent antiviral antibiotic, oxetanocin A, which has an oxetanosyl-N-glycoside linkage to an adenine moiety. However, the oxetanocin A productivity of the original strain was unstable and low. In this study, oxetanocin A productivity and resistance was shown to be lost simultaneously when a 51.5-kb plasmid, pOXT1,was cured during cultivation. The deficiency of oxetanocin A productivity and resistance was restored by re-introduction of the pOXT1 plasmid into the cured strain. By a cloning experiment it was shown that a 6.8-kb BglII-D fragment of the pOXT1 plasmid was responsible for oxetanocin A productivity and resistance.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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Tomita Koji
Bristol-myers Squibb Research Institute
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Takahashi Hideo
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Takahashi H
Tokyo Inst. Technol. Yokohama Jpn
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Kawamura F
Institute Of Molecular And Cellular Biosciences The University Of Tokyo:(present Address)lab. Molecu
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Kawamura Fujio
Institute Of Applied Microbiology The University Of Tokyo
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Kawamura Fujio
Institue Of Applied Microbiology The University Of Tokyo
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MORITA Makoto
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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TOMITA Katsuhisa
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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ISHIZAWA Masaru
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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TAKAGI Kenkichi
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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MORINO Tomio
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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Morita M
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Morino T
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Morino Tomio
Research And Development Division Pharmaceutical Group Nippon Kayaku Co. Ltd.
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Tomita Katsuhisa
Research Institute Fancl Co.
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Takahashi Hideo
Institute Of Applied Microbiology The University Of Tokyo
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Ishizawa Masaru
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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TAKAHASHI Hideo
Institue of Applied Microbiology, The University of Tokyo
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