Establishment of a Host-vector System in Bacillus megaterium Strain NK84-0128, an Oxetanocin A Producer
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概要
- 論文の詳細を見る
A host-vector system was constructed in Bacillus megaterium strain NK84-0128; an oxetanocin A producer. The replication origin of an endogeneous plasmid, P-4, was used to construct a potential plasmid vector, pSM5, which had a chloramphenicol resistance gene as a selective marker. Plasmid transformation by a protoplast method was used in B. megaterium strain NK84-0128. The maximum transformation frequency attained with the pSM5 plasmid was 2.0×10^4 cfu/μg DNA.
- 社団法人日本農芸化学会の論文
- 1996-06-23
著者
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Tomita Koji
Bristol-myers Squibb Research Institute
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Sato Sachiko
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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MORITA Makoto
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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TOMITA Katsuhisa
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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MORINO Tomio
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd.
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Morita M
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Morino T
Research And Development Division Pharmaceuticals Group Nippon Kayaku Co. Ltd.
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Morino Tomio
Research And Development Division Pharmaceutical Group Nippon Kayaku Co. Ltd.
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Tomita Katsuhisa
Research Institute Fancl Co.
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