Restricted Transcription from Sigma H or Phosphorylated Spo0A Dependent Promoters in the Temperature-sensitive secA341 Mutant of Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
The temperature-sensitive secA341 mutation of Bacillus subtilis affects sporulation and sporulation-associated events as well as protein secretion and cell septation. With lacZ or bgaB fusion genes, we examined the expression of the early sporulation genes in the mutant strain. Transcriptional expression of σ^H dependent kinA, spo0A (Ps), phrC, spoVG, and citG (p2) genes was blocked by the secA341 mutation at 37℃. On the other hand, neither repression of the abrB gene nor induction of the spo0H (σ^H) gene was affected. Active RNA polymerase containing σ^H was, however, found to be produced in the mutant cells. Expression of the phosphorylated Spo0A dependent spoIIG operon was also blocked. Thus the secA341 mutation blocks some step(s) or factor(s) required for σ^H-dependent transcription in vivo.
- 社団法人日本農芸化学会の論文
- 1998-09-23
著者
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Takahashi Hideo
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Sadaie Yoshito
National Institute of Genetics
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Asai Kei
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Sadaie Yoshito
Radioisotope Center National Institute Of Genetics
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Kawamura F
Dep. Of Life Sci. Coll. Of Sci. Rikkyo Univ.
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Kawamura Fujio
Institute Of Applied Microbiology The University Of Tokyo
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Kawamura Fujio
Institue Of Applied Microbiology The University Of Tokyo
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Kobayashi Yasuo
Laboratory of Molecular Biology and Microbial Chemistry, Tokyo University of Agriculture and Technol
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FUJITA Masaya
Radioisotope Center, National Institute of Genetics
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ASAI Kei
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Asai Kei
Area Of Biochemistry And Molecular Biology Division Of Life Science Graduate School Of Science And E
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Fujita Masaya
Radioisotope Center National Institute Of Genetics
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Takahashi Hideo
Institute Of Applied Microbiology The University Of Tokyo
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Kobayashi Yasuo
Laboratory Of Molecular Biology And Microbial Chemistry Tokyo University Of Agriculture And Technolo
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TAKAHASHI Hideo
Institue of Applied Microbiology, The University of Tokyo
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