Isolation and Characterization of Sporulation-Initiation Mutation in the Bacillus subtilis prfB Gene
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概要
- 論文の詳細を見る
A Bacillus subtilis prfB45 mutant grew at 42 °C, but its sporulation was severely defective at 37 °C. Sporulation-specific induction of kinA, spo0A, and spo0H genes was inhibited in the mutant. The effects of temperature up-shift and down-shift on sporulation of the prfB45 mutant was observed at an early stage of sporulation. UGA readthrough frequency at non-permissive temperatures for sporulation was higher in the mutant than in the wild-type strain. Temperature-sensitive sporulation of the prfB45 mutant was suppressed by mutations in rpsL coding for S12 of ribosomes, required for accurate termination of translation. Additionally, spontaneous second-site mutations that suppressed the sporulation phenotype of the prfB45 strain were found in the rpoB gene. These results suggest that accurate termination of translation is required for proper initiation of sporulation.
- 社団法人 日本農芸化学会の論文
- 2007-02-23
著者
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Ochi Kozo
National Food Research Institute
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Ochi Kozo
National Food Res. Inst.
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Ochi K
National Food Res. Inst.
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Sadaie Yoshito
National Institute of Genetics
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Asai Kei
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Kawamura F
Dep. Of Life Sci. Coll. Of Sci. Rikkyo Univ.
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Sadaie Yoshito
Area of Biochemistry and Molecular Biology, Division of Life Science, Graduate School of Science and
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Asai Kei
Area of Biochemistry and Molecular Biology, Division of Life Science, Graduate School of Science and
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INAOKA Takashi
National Food Research Institute
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KAWAMURA Fujio
Department of Life Science, College of Science, Rikkyo University
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Nanamiya Hideaki
Department Of Life Science College Of Science Rikkyo University
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Asai Kei
Area Of Biochemistry And Molecular Biology Division Of Life Science Graduate School Of Science And E
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Asai Kei
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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NANAMIYA Hideaki
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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