Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phage
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概要
- 論文の詳細を見る
Mutational inactivation of both nonA and nonB genes are required for the permissiveness of Bacillus subtilis Marburg cells to infection by phage SP10. By transformational analysis of the nonA strain with DNAs from gently lysed protoplasts carrying the integrative plasmid pMUTIN (em) insertions in every 20 kb along the whole chromosome, we have identified the nonA to be the cured state of endogenous prophage SPβ. Direct DNA sequencing, on the other hand, revealed one nonsense mutation of nonB in ydiR, which is a component gene of the intrinsic restriction system BsuMR of B.subtilis Marburg. Introduction of the wild type ydiR into the nonB strain at aprE locus resulted in complementation of nonB. Furthermore, as the SP10 genome was found to possess multiple BsuM target sites, it is considered that SP10 can infect and multiply in B.subtilis cells, which are SPβ free and possess a defective BsuMR restriction system.
- 日本遺伝学会の論文
- 2004-12-25
著者
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松岡 達
京都大学大学院医学研究科
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MATSUOKA Satoshi
Department of Physiology and Biophysics, Graduate School of Medicine, Kyoto University
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Matsuoka Satoshi
京都大学 医学研究科循環器外科
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Arai Tomomi
Department of Physics, Faculty of Science and Engineering, Waseda University
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SADAIE Yoshito
Department of Molecular Biology, Faculty of Science, Saitama University
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ASAI Kei
Department of Molecular Biology, Faculty of Science, Saitama University
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Sadaie Yoshito
National Institute of Genetics
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Matsuoka Satoshi
Department Of Physiology And Biophysics Graduate School Of Medicine Kyoto University
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Asai Kei
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Arai Tomomi
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Sadaie Yoshito
Department Of Biochemistry And Molecular Biology Graduate School Of Science And Engineering Saitama
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Kawamura F
Dep. Of Life Sci. Coll. Of Sci. Rikkyo Univ.
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MURAYAMA Rikinori
Department of Life Science, College of Science, Rikkyo University
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KAWAMURA Fujio
Department of Life Science, College of Science, Rikkyo University
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Asai Kei
Area Of Biochemistry And Molecular Biology Division Of Life Science Graduate School Of Science And E
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Murayama Rikinori
Department Of Life Science College Of Science Rikkyo University
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Matsuoka Satoshi
Department Of Biochemistry And Molecular Biology Graduate School Of Science And Engineering Saitama
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Matsuoka Satoshi
Area Of Biochemistry And Molecular Biology Division Of Life Science Graduate School Of Science And E
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Asai Kei
Department Of Biochemistry And Molecular Biology Faculty Of Science
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Asai Kei
Departement of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
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Sadaie Yoshito
Departement of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
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