Molecular cloning of a Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion.

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概要

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• The wild type div-341+ gene of Bacillus subtilis was cloned in a temperate phage ρ11, and was recloned in a smaller temperate phage φ105. The resulting Div+ transducing phage carried a 3 kilobase Cfr13I digested chromosomal fragment which showed Div+ transforming activity and contained the whole div-341+ gene which is involved in cell division, sporulation, exoenzyme secretion, competent cell formation, and autolysis. A partial restriction map of the fragment was established. The merodiploid system of the div-341+ gene, wild type gene on the phage genome and mutant gene on the chromosome, resulted in the suppression of mutant phenotypes and indicated that the wild type div-341+ gene is dominant over mutant gene.

• 日本遺伝学会の論文

著者

• Sadaie Yoshito

  Radioisotope Center National Institute Of Genetics

• Sadaie Yoshito

  Radioisotope Center, National Institute of Genetics

関連論文

• Restricted Transcription from Sigma H or Phosphorylated Spo0A Dependent Promoters in the Temperature-sensitive secA341 Mutant of Bacillus subtilis
• Promoter Selectivity of the Bacillus subtilis RNA Polymerase σ^A and σ^H Holoenzymes
• Feedback Loops Involving Spo0A and AbrB in In Vitro Transcription of the Genes Involved in the Initiation of Sporulation in Bacillus subtilis
• Molecular cloning of a Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion.
• Feedback Loops Involving Spo0A and AbrB in In Vitro Transcription of the Genes Involved in the Initiation of Sporulation in Bacillus subtilis.

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