In Vitro Transcriptional Analysis of the Cytochrome P-450cam Hydroxylase Operon
スポンサーリンク
概要
- 論文の詳細を見る
To characterize the promoters of cytochrome P-450cam hydroxylase operon (camDCAB) and the repressor gene (camR), in vitro run-off transcription assays were performed using RNA polymerase (RNAP) holoenzyme reconstituted with the core enzyme and the σ^<70> protein of Pseudomonas aeruginosa. Both the mRNAs of camDCAB and camR were accurately transcribed from the respective promoter by the reconstituted RNAP holoenzyme. Both the transcriptions were repressed by CamR protein and the repressions were suppressed by D-cam-phor, consistent with the regulation in P. putida. These results suggest that the RNA polymerase containing σ^<70> recognizes the promoter of camDCAB as well as that of camR.
- 公益社団法人日本薬学会の論文
- 1999-10-15
著者
-
Fujita M
Radioisotope Center National Institute Of Genetics
-
ARAMAKI Hironori
Department of Molecular Biology, Daiichi College of Pharmaceutical Sciences
-
SAGARA Yasuhiro
Department of Medical Biology, Kochi Medical School
-
Aramaki Hironori
Department Of Molecular Biology Daiichi College Of Pharmaceutical Sciences
-
Aramaki Hironori
Department Of Microbiology Daiichi College Of Pharmaceutical Sciences:faculty Of Pharmaceutical Scie
-
Fujita M
Jcr Pharmaceuticals Co. Ltd. Kobe Jpn
-
FUJITA Masaya
Radioisotope Center, National Institute of Genetics
-
Fujita Masaya
Radioisotope Center National Institute Of Genetics
-
Sagara Yasuhiro
Department Of Medical Biology Kochi Medical School
関連論文
- Transport of Nattokinase across the Rat Intestinal Tract
- Role of Glucocorticoid Receptor in the Regulation of Cellular Sensitivity to Irinotecan Hydrochloride
- Different Effects of Carboxy-Terminal Deletion in the Adrenodoxin Molecule on Cytochrome c and Acetylated Cytochrome c Reductions
- Thrombolytic Effect of Nattokinase on a Chemically Induced Thrombosis Model in Rat
- Feast/famine regulatory proteins of a multiple drug resistant, Pseudomonas aeruginosa
- Cloning and Sequence Analysis of a Full-Length cDNA of Rat Adrenodoxin
- In Vitro Transcriptional Analysis of the Cytochrome P-450cam Hydroxylase Operon
- Transcription Regulation by Feast/Famine Regulatory Proteins, FFRPs, in Archaea and Eubacteria
- ON THE STRUCTURE AND GENE EXPRESSION OF CYTOCHROME P450cam AND ITS RELATING GENES CARRIED BY CAM PLASMID IN PSEUDOMONAS PUTIDA
- Cell-Cycle-Dependent Pharmacology of Methotrexate in HL-60
- Restricted Transcription from Sigma H or Phosphorylated Spo0A Dependent Promoters in the Temperature-sensitive secA341 Mutant of Bacillus subtilis
- Cannabidiol-2′,6′-dimethyl Ether as an Effective Protector of 15-Lipoxygenase-Mediated Low-Density Lipoprotein Oxidation in Vitro
- CONTROL OF EXPRESSION OF THE CYTOCHROME P-450cam OPERON AND PURIFICATION OF THE cam REPRESSOR (CAMR) OF PSEUDOMONAS PUTIDA PpG1
- Nucleotide sequences of the plastid and nuclear chromosome I of the unicellular red alga Cyanidioschyzon merolae
- Promoter Selectivity of the Bacillus subtilis RNA Polymerase σ^A and σ^H Holoenzymes
- Determination of the complete genomic DNA sequence of Thermoplasma volcanium GSS1
- Feedback Loops Involving Spo0A and AbrB in In Vitro Transcription of the Genes Involved in the Initiation of Sporulation in Bacillus subtilis
- Overproduction in Escherichia coli and Characterization of the Precise Mature Form of Rat Adrenodoxin
- Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ^9-tetrahydrocannabinol in human breast cancer cells
- (-)-Xanthatin induces the prolonged expression of c-Fos through an N-acetyl-L-cysteine (NAC)-sensitive mechanism in human breast cancer MDA-MB-231 cells
- Feedback Loops Involving Spo0A and AbrB in In Vitro Transcription of the Genes Involved in the Initiation of Sporulation in Bacillus subtilis.
- Down-regulation of cyclooxygenase-2 (COX-2) by cannabidiolic acid in human breast cancer cells