Thrombolytic Effect of Nattokinase on a Chemically Induced Thrombosis Model in Rat
スポンサーリンク
概要
- 論文の詳細を見る
Nattokinase is a new fibrinolytic enzyme which cleaves directly cross-linked fibrin in vitro. In this study, we investigated the thrombolytic effect of nattokinase on a thrombus in the common carotid artery of rat in which the endothelial cells of the vessel wall were injured by acetic acid. When a section of occluded vessel was stained for CD61 antigen by immunofluorescence utilizing a monoclonal antibody, the antigen was localized around the surface of the occluded blood vessels. This result suggests that the occlusive thrombosis was caused by platelet aggregation. In addition, thrombolysis with urokinase (UK ; 50000IU/kg, i.v.) or tissue plasminogen activator (tPA ; 13300IU/kg, i.v.) in our model was observed to restore the blood flow over a 60 min monitoring period. The results indicate that our chemically induced model is useful for screening and evaluating a thrombolytic agent. We evaluated the thrombolytic activity of nattokinase using this model and compared it with fibrino (geno) lytic enzyme, plasmin or elastase. On a molar basis, the recovery of the arterial blood flow with nattokinase, plasmin and elastase were 62.0±5.3%, 15.8±0.7% and 0%, respectively. The results indicate that the thrombolytic activity of nattokinase is stronger than that of plasmin or elastase in vivo.
- 公益社団法人日本薬学会の論文
- 1995-10-15
著者
-
藤田 貢
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd.
-
洪 卿秀
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd.
-
伊東 八重
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd.
-
仮家 公夫
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
-
西室 悟司
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd.
-
西室 悟司
Biotechnology Research Laboratories Jcr Pharmaceuticals Co. Ltd.
-
藤井 理恵
Department of Earth Sciences, Graduate School of Social and Cultural Studies, Kyushu University
-
藤井 理恵
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
-
Fujita M
Radioisotope Center National Institute Of Genetics
-
Ito Y
Kinki Univ. Osaka Jpn
-
Kariya K
Kobe Gakuin University Faculty Of Pharmaceutical Sciences
-
仮家 公夫
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
-
洪 卿秀
Biotechnology Research Laboratories Jcr Pharmaceuticals Co. Ltd.
-
伊東 八重
Biotechnology Research Laboratories Jcr Pharmaceuticals Co. Ltd.
-
Fujii Rie
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe-gakuin University
-
Fujita M
Jcr Pharmaceuticals Co. Ltd. Kobe Jpn
-
藤田 貢
Biotechnology Research Laboratories Jcr Pharmaceuticals Co. Ltd.
関連論文
- Transport of Nattokinase across the Rat Intestinal Tract
- 古カトマンズ湖ボーリング計画
- Mechanism of Potentiation of the Carcinostatic Activity of 8-Aza-guanine by 4 (or 5)-Aminoimidazole-5 (or 4)-carboxamide Analogs
- Studies on the Ethylhydroperoxide-Supported Oxidation of 1,4-Diazabicyclo[2.2.2]octane by Chloroperoxidase
- Synthesis and Anti-tumor Activity of Tenuazonic Acid Analogues
- A Parallel Comparison of Age-Related Peripheral Nerve Changes in Three Different Strains of Mice
- Comparison of Age-Related Peripheral Nerve Changes in Mice Housed in Either Plastic Cages with Sawdust-Covered Solid Flooring or Wire-Mesh-Floor Cages
- 21B-03-1 Clinical usefulness of 1,5-anhydroglucitol, fructosamine and hemoglobin A_ in mice
- Thrombolytic Effect of Nattokinase on a Chemically Induced Thrombosis Model in Rat
- EFFECTS OF KOJIC ACID ON THYROIDAL FUNCTIONS IN RATS BY SINGLE-DOSE ADMINISTRATION AND IN CULTURED RAT THYROID CELLS (FRTL-5 CELLS)
- STUDIES ON THYROID FUNCTION IN RATS SUBJECTED TO REPEATED ORAL ADMINISTRATION WITH KOJIC ACID
- 21D-03-2 Action Mechanism of Kojic Acid on Rat Thyroid Function
- Effect of Kojic Acid on Rat Thyroid Gland Administered Orally for 4 Weeks
- Decrease in Myeloperoxidase during Differentiation of Bone Marrow Cells by Colony-Stimulating Factor
- SPONTANEOUS AGE-RELATED PERIPHERAL NEUROPATHY IN B6C3F1 MICE
- In Vitro Transcriptional Analysis of the Cytochrome P-450cam Hydroxylase Operon
- 22B-09-4 Spontaneous age-related peripheral neuropathy in B6C3F1 mice.
- EFFECT OF COMPOUNDS AFFECTING THE ADRENERGIC MECHANISM ON CELL GROWTH AND DIVISION OF TETRAHYMENA PYRIFORMIS W
- VARIANCE OF THE GROWTH OF TETRAHYMENA BY THE COMPOUNDS WHICH AFFECT ON THE ADRENERGIC MECHANISM
- Chloroperoxidase-Catalyzed Oxidation of Aminopyrine
- Promoter Selectivity of the Bacillus subtilis RNA Polymerase σ^A and σ^H Holoenzymes
- 毒科学におけるアポトーシス